Last updated on Mar 2022
of 30 minutes to allow sufficient time for it to warm up and run properly. The lamp has
an enforced cooldown of 300 seconds (5 minutes) after shutdown where the lamp
cannot be switched on until the 300 seconds is up.
•
Check the timer on the power box to ensure the mercury lamp does not run past 2,000
hours. The timer indicator on this particular lamp power box blinks when the timer
crosses 1,900 hours.
•
Do not look directly at the MHL when it is switched on as it can damage your eyes.
•
Do not attempt to remove the light guide from the MHL,
especially
when it is on.
Direct emission of the MHL may set off fires.
•
If there are no users in the next 30 mins, MHL must be turned off.
•
Never attempt to change a bulb yourself. Please contact facility staff when you notice
that a bulb has reached between 2,000
–
2,500 hours or is not functioning properly.
•
Important!
If you hear an explosion and see the burner indicator turn red from blue,
save your work and
evacuate immediately
.
2. Training
Use of confocal microscopy and the online booking system is authorized by facility staff
only. All users must attend the confocal training session conducted by the Bioimaging facility
staff before they are allowed to access the confocal system.
2.1 To arrange a training session:
•
Please email Bioimaging Facility (
) for a training session.
•
Fill in the necessary particulars in the form here:
http://microscopy.tll.org.sg/pages/conf_training_form.html
(E.g. Lab, PI, sample, and a brief description of your project)
•
We will help you identify the most appropriate microscope for your imaging needs.
•
We will then arrange training session according to your availability within a week.
•
Training sessions usually last 1 -
3 hours depending on users’ previous experience.
•
Users receiving training are highly encouraged to bring their own samples so that
staff can adjust trainings to your imaging needs, but if not possible, staff have their
own samples that they can work with.
Once you have completed the training session, we will grant you access to both the confocal
PC and the online booking system. Please keep in mind that only bioimaging facility
members are allowed to conduct the training. None of your lab members are allowed to
conduct the training for you.
If you need a refresher or some specific advice on anything microscopy related, please
approach any member of the Bioimaging Facility for assistance/help.
We also offer training on the various types of image analysis and image processing software
available here at TLL, including ImageJ/Fiji, Huygens and Imaris.
