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Last updated on Mar 2022 

3. Online Booking and System Access 

 

Booking of all Light microscopes prior to use is 

COMPULSORY 

through

 

microscopy 

resource booking via TLL intranet 

(

https://intranet.tll.org.sg/App/tll_intranet/booking_searches

)

 

 

Users are only allowed to book the confocal system that they have received training 
on. If they wish to book other confocal system, they have to receive a separate 
training. 

 

Users are entitled to advance bookings of up to 2 weeks. They are advised to plan their 
experiments accordingly to avoid any disappointments. 

 

During Office Hours (

Weekdays from

 

8.30am to 6pm

)  

 

Each user is entitled to a 

MAXIMUM of 2 bookings

 per system per week. 

 

Users whom are trained on multiple systems are entitled to a maximum of 3 
bookings per week but it has to be shared across the systems that they have 
received training on.  

 

Each booking must not exceed 3 hours.  

 

If users have utilized all their entitled bookings for the week, 

“24Hr Rule” 

can be 

applied where they can book the system in less than 24 hours in advance according 
to its availability. If extra slots are booked within the 24-hour period, a note of 

“24Hr Rule”

 should be made in the booking description.  

 

During Non - Office Hours (

Weekdays after 6pm, Sat, Sun & Public holidays

 

If users require more slots in a particular week, they can book on weekdays during 
non-peak hours (

after 6pm

), on weekends (

Sat and Sun

) and on public holidays. 

 

If extra time is required, bookings can be extended out of peak hours

 (E.g. 3 

 

7pm).

 

 

Bookings exceeding these limits are subjected to cancellation without prior warning.  

 

Simultaneous bookings of two or more different systems are not allowed. Multiple 
bookings for the same system on the same day during office hours is strictly 
prohibited. 

 

If users cannot attend a booked session for any reason, it is 

COMPULSORY

 that they 

cancel their booking through TLL intranet and email 

(

confocal@tll.org.sg

)

 to announce the 

availability. If they are the last users for the day, they need to check if the system has 
been switched off completely. 

 

If users are swapping a session with another user, they must change the booking 
details accordingly. 

 

Under any circumstances, users are not allowed to make bookings on behalf of other 
people. Users who have received training but are yet to gain access to microscopy 
resource booking may approach TLL Bioimaging department for booking assistance if 
they need to use it urgently.  

 

If a user fails to show up within the first 30 minutes of their booking, the slot is 
forfeited and is free for any user to use it.  

 

If any users violate any of these Booking rules, users will be subjected to the 3 strikes 
policy. 

 

 

Summary of Contents for FV3000

Page 1: ...oking and System Access 4 3 1 Acknowledgements 5 4 Operation Procedures 5 4 1 Switching ON Protocol 5 4 2 Objective lens 6 4 2 Software Initiation 7 4 3 Setting up directory to save images 8 4 4 Laser...

Page 2: ...Immersion Oil Modern immersion oil has no known hazards to human beings so far yet they can cause discomfort to a person if the immersion oil has been left on skin for too long or inhaled Therefore pl...

Page 3: ...taff before they are allowed to access the confocal system 2 1 To arrange a training session Please email Bioimaging Facility bioimaging tll org sg for a training session Fill in the necessary particu...

Page 4: ...er 6pm Sat Sun Public holidays If users require more slots in a particular week they can book on weekdays during non peak hours after 6pm on weekends Sat and Sun and on public holidays If extra time i...

Page 5: ...RT TIMING b Switch on the Master switch on the wall 1 PC 2 Wait until windows icon pops up c Switch on Stage controller box 3 CBH controller 4 d Optional Turn on the Metal Halide lamp 5 if needed e Tu...

Page 6: ...t to 10x objective or lower then click YES Cleaning the stage will take some time b Otherwise click NO to skip cleaning 4 2 Objective lens Objective lens Immersion media 1 25x 0 04 Plan Apochromat Air...

Page 7: ...n the stage mechanism This adds time to the initialization process Do not place anything on the stage mechanism until the initialization process is complete If you are not the first user of the week C...

Page 8: ...y to save all your images Please save in your folder You have several different options on where you can save your images Option 1 D or E Users shaalini create your own folder BPAE cells oir Do keep i...

Page 9: ...oduce intense background noise SD keep HV within 600 800 More than 800 will contribute to intense background noise Under PMT settings tab Mode Select VBF Average average a given number of scans to get...

Page 10: ...to zoom images only in the center Ideal zoom which satisfy the Nyquist limit can be achieved by zooming till the red arrow any zoom beyond it will result in poor resolution and result in more photoble...

Page 11: ...the laser power until red pixels disappear Increase Offset until a dusting of blue pixels in the background are observed Click LSM start to capture an image 4 7 How to do a Z stack for a 3D Image xyz...

Page 12: ...your timelapse experiment Interval FreeRun continues to capture images until it has completed your selected cycle You could always choose an interval of your choice 4 9 Reuse previous settings and ex...

Page 13: ...h the sample and return the stage to central position d Carefully clean the objective lens with Whatman lens tissue using 100 ethanol e Switch lens back to 10x 0 3 objective lens f Exit the FV31S SW s...

Page 14: ...ission please contact Bioimaging Group No stage movement Check the coarse fine movement setting near the focus and xy position knobs It is a physical button that has no electronic indication elsewhere...

Page 15: ...id white or coloured light sometimes red light might be indicative of error III Restart the main controller right at the bottom if none of the steps taken work especially if the machine has been left...

Page 16: ...and cannot be recovered Restart computer terminal Startup popup error code LAST_OPERATION_ NONCOMPLETED The last user did not shut down the program properly or the program encountered a fatal error du...

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