12
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic
observation method of this microscope. The operating procedures for phase contrast observation and DIC observation
will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 32.
For the fluorescence observation, refer to the separate instruction manual entitled “Manual/Motorized Reflected Fluores-
cence System”.
(Controls Used)
(Page)
Set the main switch to “
I
” (ON).
@Main switch: “
I
” (ON)
(P. 14)
²Lamp ON-OFF button: ON
(P. 14)
³Light intensity control knob
(P. 14)
Disengage the DIC slider and
analyzer from the light path.
Disengage the filter other than the
frost filter from the light path.
|Filter pocket
(P. 23/26)
Select the (observation) light path with the
light path selector lever.
ƒLight path selector lever
(P. 15)
Place the specimen on the stage.
…X-axis and Y-axis knobs
(P. 19)
Engage a 10X objective in the light path.
†Revolving nosepiece
Bring the specimen into focus.
‡Coarse/fine adjustment knobs
(P. 16)
Adjust the brightness.
³Light intensity control knob
(P. 14)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
ŠBinocular observation tube
(P. 20)
‰Diopter adjustment ring
(P. 20)
‹Condenser height adjustment knob
*
(P. 27/
28)
ŒCondenser centering knobs
*
(P. 27/28)
™Field iris diaphragm lever
*
(P. 24)
Engage the objective to be used in the light
path and bring the specimen in focus.
†Revolving nosepiece
‡Coarse/fine adjustment knobs
(P. 16)
Adjust the aperture iris and field iris diaphragms.
šAperture iris diaphragm lever
(P. 29)
™Field iris diaphragm lever
*
(P. 24)
Engage the required filters.
|Filter pocket
(P. 23/26)
Adjust the brightness.
³Light intensity control knob
(P. 14)
Start observation.
*
This operation is not required when the IX2-ILL30 illumination column is used.
