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Problem
Cause
Remedy
Page
f ) Visibility of observed image is poor.
· Image is not sharp.
· Contrast is poor.
· Details are poorly visible.
Objective in use is not designed for
UIS2 series.
Replace with an objective designed
for UIS2 optics.
-
Correction collar on the objective
equipped with correction collar is not
adjusted.
Adjust the correction collar to acquire
the best contrast.
32
Front lens of the objective is dirty.
Clean the objective.
6
The immersion oil appropriate with
an oil immersion objective is not
used.
Use Olympus immersion oil with the
oil immersion objective, Olympus sili-
cone oil with the silicone immersion
objective and water with the water
immersion objective.
31
Immersion oil contains bubbles.
Remove bubbles.
-
Inappropriate slide or cover glass
thickness.
Replace with glass of appropriate
thickness.
-
Glass components (condenser,
objective, eyepieces, culture vessels,
etc.) are dirty.
Clean thoroughly.
6
Ring slit and phase plate are not
centered.
Center them correctly.
36
A plastic culture vessel is used.
Replace the plastic culture vessel with
a glass vessel.
37
g) A part or one side of the field of
view is blurred.
Specimen is tilted with respect to the
stage.
Place the specimen correctly on the
stage and secure it with the stage
clip.
18
h) Field of view of one eye does not
match that of the other.
The interpupillary distance is incor-
rect.
Adjust the interpupillary distance.
23
Incorrect diopter adjustment.
Adjust the diopter.
23
You are not accustomed to parallel
optical axis.
When looking into the eyepieces, do
not stare at image from the beginning
but see the overall field of view. It is
sometimes recommended to turn
your eyes away from the eyepieces,
look far off and look into the eye-
pieces again.
-
i ) After focused on the specimen, it
will be defocused over time.
The thermal drift occurs due to the
fluctuation of the room temperature.
Keep the room temperature constant
as much as possible.
-
When using IX3-SVR, the thermal
drift could be small by loosening the
holder fixing screws (4 pcs.) once and
tightening them lightly,
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