Olympus IX81, Instructions Manual

Page 1: ...d Research Microscope Model IX81 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference ...

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Page 3: ...an objective with a short working distance let the motorized revolving nosepiece escape once before pressing the objective switching button 1 Voltage Indication 2 Light Path Selection 3 Magnification Change 4 Motorized Revolving Nosepiece 5 Frame Clamping Plate 1 Adjusting the Interpupillary Distance 2 Adjusting the Diopter 3 Using the Eye Shades 4 Using Eyepiece Micrometer Disks 5 Selecting the L...

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Page 5: ...ted and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may ca...

Page 6: ...corporating the power supply and Hand Switch IX2 Software for PC CD ROM Model name IX2 BSW Ver 01 03 or later Methods of PC control of functions Fluorescence system for IX2 Reflected light fluorescence observation U FWT FWR FWO Motorized filter wheels The U FWT cannot be used with this microscope Fig 1 Precautions When Unpacking the Microscope Releasing the Transport Lock of the Motorized Revolvin...

Page 7: ...n the microscope frame in either direction or press any of the FOCUS control buttons F C and ESC except during PC communications When the main switch of the IX2 UCB control box is set to I ON the focusing operation starts automatically as part of initialization this operation consists of temporary raising and then returning to the original position of the objective If any of the operations mention...

Page 8: ...ce 10 cm or more around and in particular above the lamp housing 9 When installing the microscope route the power cord away from the lamp housing Should the power cord come in contact with the hot lamp housing the power cord could melt and cause electric shock Fig 3 3 Designated Bulb 12V100WHAL L PHILIPS 7724 11 Always use the power cord provided by Olympus If no power cord is provided please sele...

Page 9: ...ubjected to direct sunlight high temperature and humidity dust or vibrations For operating conditions see Chapter 8 SPECIFICATIONS on page 48 3 An intermediate attachment with a thickness of up to 60 mm can be mounted between the microscope frame and binocular observation tube U BI90CT U BI90 If the U BI90CT is used together with an intermediate attachment the image may be cut off or obscured 4 Th...

Page 10: ...available absolute alcohol Since the absolute alcohol is highly flammable it must be handled carefully Be sure to keep it away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched on or off Also remember to always use it only in a well ventilated room 2 Do not attempt to use organic solvents to clean the non optical components of micros...

Page 11: ...U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU Motorized Revolving Nosepiece Fixed on the microscope frame Microscope Frame IX81S1F 3 IX81S8F 3 IX81F 3 Used together with IX2 PRS8 or IX2 PRS1 Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Focus Adjustment Knob Interface U IFFH Focus Adjustment Knob Unit U FH Hand Switch U HSTR2 Transmitted Illumination Column IX2 ILL100 Transmit...

Page 12: ...erture iris diaphragm lever Indicator plate pockets Light path selector button Page 13 Focusing buttons Page 15 Auxiliary button Transmitted light ON OFF button Page 13 Lamp voltage indicator Page 13 Light intensity control buttons Page 13 Focusing knob Page 15 Detachable IX2 ILL100 Condenser height adjustment knob Page 22 Stage center plate Page 16 IX81S1F 3 IX81S8F 3 IX81F 3 Y axis knob Page 17 ...

Page 13: ...r Observation Tube U TBI90 Intermediate Attachment Tube IX ATU storage position Rear panel Side port Transport lock knob Be sure to remove this before use Allen wrench and optical element centering knob x 2 storage position Rear panel Interpupillary distance adjustment scale Page 18 CT turret Page 19 Focus ring Page 19 Focusing knob Page 15 Coarse fine adjustment switching button Page 15 Objective...

Page 14: ...m lever Page 23 This is a manually controlled condenser Re fer to the separate instruction manual for de tails Plain Stage IX2 SP Mechanical Stage IX MVR Cross Stage IX SVL2 UCD Adapter IX ADUCD LWD Universal Condenser IX2 LWUCD ULWCD Condenser IX ULWCD Universal Condenser U UCD8 On both sides Y axis knob X axis knob Turret Page 23 Turret Page 23 This condenser is manually operated IX MVR mounting...

Page 15: ...Focus Adjustment Knob Unit U FH Abbreviations Symbols Function Note Fine Coarse switching Set cancel photo voltage Microscope frame U FH switching of focus adjustment knob Z focusing motor ON OFF OFF Electrical noise reduction Escape return objective Shutter IN OUT Condenser top lens IN OUT Not used with the IX81 Up down operation for brightness ad justment objective etc The function name can be w...

Page 16: ...d observation by pressing the BF button and select the observation light path 4BF observation button 5Light path selector button P 13 Place the specimen on the stage 6X axis and Y axis knobs P 17 Bring the specimen in focus 8Focusing knob dial P 15 Focus adjustment knob or focusing button Adjust the brightness 9Light intensity control buttons P 13 Adjust the interpupillary distance Adjust the diop...

Page 17: ...setups at the moment the PC is started up Controls with numbers inside are not available when the U FH focus adjustment knob unit is in use 1 2 3 4 5 6 7 8 9 a b c d e f 8 Make a photocopy of the observation procedure pages and post it near your microscope 8 ...

Page 18: ...s driven but the illumination intensity does not vary even if this occurs 3 The marking 5 is the reference position indicating 9 V which is the suitable intensity for color photography using LBD filter 2 Light Path Selection Fig 4 The light path selector button 6 allows for light path switching between the observation and side port paths The selected light path can be confirmed with the LED on the...

Page 19: ... appropriate button on the U HSTR2 hand switch or a button assigned by the PC When the IX2 BSW software Ver 01 03 or later is started up the revolving nosepiece escapes to avoid interference with the stage as the objective is switched and returns to the original position after objective changing It is basically inhibited to attempt to rotate a motorized revolving nosepiece manually However it can ...

Page 20: ...djustment stroke is 0 5 mm per turn with the initial setting but this can be changed using the IX2 BSW software To select the desired adjustment stroke press the coarse fine adjustment switching button 2 Coarse adjustment 1 mm per turn Fine adjustment 0 5 mm per turn 3 Focusing UP DOWN buttons Fig 7 The focusing speed available with these buttons is coarse 3 mm sec only The focusing speed can be c...

Page 21: ... specimen holder 1 and slide the microtiter plate into the holder frame Gently release the curved finger to clamp Fig 9 To secure other vessels than microtiter plates various optional holders are available A Terasaki plate holder 2 is available for holding Terasaki plates 72 well 60 well When using this it is necessary to replace the stage scales with those provided with the plate holder Petri dis...

Page 22: ...the stage for electrophysiological experiments etc Prepare a grounding wire 1 and one M4 screw 2 and attach the ground ing wire as shown in Fig 12 The screw hole may sometimes be stuck by paint etc In such a case screw in the M4 screw a few times to expose the metallic thread inside the screw hole and improve the contact before attaching the grounding wire firmly 4 Adjusting the X Axis Y Axis Knob...

Page 23: ...stment ring 2 on the left eyepiece to bring the specimen into focus Fig 15 When rotating the diopter adjustment ring of an eyepiece hold the lower part of the eyepiece with the other hand Operation When Using Finder Eyepieces with the U TR30H 2 The finder eyepieces cannot be mounted to the eyepiece sleeves of a binocular observation tube 1 Looking through the left eyepiece with your left eye rotat...

Page 24: ...cular 20 TV photo 80 Observation of bright specimens photomicrography TV observation Pulled out TV photo 100 Photomicrography TV observation 6 Using the CT Turret U BI90CT Only Fig 20 1 To use the CT turret 1 rotate the knurled ring with a finger to select the setting corresponding to the indication 0 CT 0 S Set the magnification selector knob on the IX81 microscope frame to 1X Turret Indication A...

Page 25: ...orking space can be created by tilting the illumination column Even with the illumination column tilted the specimen surface will be illuminated which is convenient for rough confirmation of the specimen location or initial positioning when placing the specimen 1 Using the Allen screwdriver loosen the column tilt clamping screw 1 by turning it approximately 11 turns in the direction of the arrow 2...

Page 26: ...ile holding the mounting lever 2 of the filter holder insert a filter Fig 24 Hold the filter by its edge to avoid leaving fingerprints or smudges on the filter surfaces After the illumination has been ignited the filter will be very hot Be sure to set the main switch to OFF and allow the filter holder and filters to cool down before replacing filters 3 Engage each filter in the light path by movin...

Page 27: ...tore it in the position as shown in Fig 28 2 Insert the Allen screwdriver into the hole 3 on the condenser height adjustment knob 2 and while holding the knob turn the screwdriver in the direction of the arrow to increase the rotation tension of the knob or in the opposite direction to decrease the tension 3 After adjustment tighten the two clamping screws 1 securely Changing the Knob Position The...

Page 28: ...he condenser height adjustment knob 4 to bring the field iris diaphragm image into focus 7 While gradually opening the field iris diaphragm rotate the condenser centering knobs 5 on the condenser holder to adjust so that the field iris diaphragm image is centered in the field of view of the eyepieces 8 To check centration open the field iris diaphragm until its image inscribed the field of view No...

Page 29: ...Using the field iris diaphragm lever stop down the field iris diaphragm until its image is just inside the field of view 8 Slowly move the condenser height fine adjustment knob 1 toward the front to bring the field iris diaphragm image into focus When focusing is obtained rotate the knob clockwise to clamp it 9 While gradually opening the field iris diaphragm rotate the condenser holder s condense...

Page 30: ...m image and the objective s exit pupil When using the U BI90CT binocular observation tube the aperture iris diaphragm can be observed by setting the turret to position CT or using the U CT30 centering telescope 3 Flipping Up the Condenser Holder Fig 33 To create working space and facilitate specimen replacement microma nipulator positioning or mounting an objective through the hole on the stage fl...

Page 31: ...p Objective Series 3 Remark Oil proof cap Objective Series 3 Remark C1 C2 UPlanFl100XO3 100XO3Ph 100XO3P Provided with microscope frame C1 1 piece C2 2 pieces C1 UApo20X3 340 40X3 340 40XOI3 340 20XW3 340 40XW3 340 UPlanApo10XW3 Apo10XO3 Apo60XW3 Apo60XW3 IR 1 piece is provided with each objective UPlanFl60XOI3 60XOI3Ph 100XOI3 UPlanApo20XO3 40XOI3 40XOI3Ph 100XOI3 100XOI3Ph PlanApo60XO3 60XO3Ph 1...

Page 32: ...mmersion oil from the objective front lens by wiping with gauze slightly moistened with absolute alcohol The same procedure is applicable when using a water immersion ob jective Caution on using the immersion oil If immersion oil comes into contact with your eye or skin immediately take the following action For eye Rinse with clean water for more than 15 minutes For skin Wash with soap and water I...

Page 33: ...nFl20XPh LCPlanFl20XPh UPlanApo10XPh IX PH2 small Ph2 UPlanFl40XPh LCPlanFl40XPh LCPlanFl60XPh UPlanApo20XPh LCAch40XPh LUCPlanFl40XPh SLCPlanFl40XPh IX PH3 large Ph3 UPlanFl100XO3Ph UPlanApo40XOI3Ph UPlanApo100XOI3Ph PlanApo60XO3Ph Insert the optical element small in the 30 mm position and the optical element large in the 38 mm position For well observation it is recommended to use the IX PHC to ...

Page 34: ...PhC UPlanFLN10X2Ph UPlanFLN20XPh LUCPlanFLN20XPh PH2 Built in Ph2 UPlanFLN40XPh LUCPlanFLN40XPh LUCPlanFLN60XPh LCAchN40XPh UPlanFLN60XOIPh UIS Series Fig 36 Fig 37 2 For the U UCD8 refer to the separate instruction manual Do not engage any optical element in the BF brightfield light path 1 Place the condenser in the orientation as shown in Fig 36 loosen the detaching screws 1 and remove the cover...

Page 35: ...un able to be rotated Do not tighten the optical element centering knobs too much for this may deform the frames of the optical elements 6 Place the index 7 provided with each optical element in the index inser tion hole having the same number 9 as the number of the position 8 in which the corresponding optical element is mounted Figs 39 40 Note that the IX ULWCD does not have the index The optica...

Page 36: ...obs 5 turn the phase contrast ring slit centering screws in positions marked so that the ring slit image overlaps with the phase plate of the objective A ghost of the ring slit image may be observed In this case overlap the brightest image with the phase plate If a thick specimen is moved the ring slit image may be deviated from the phase plate and the contrast may be deteriorated In this case re ...

Page 37: ... following table in the 30 mm position and other optical element large in the 38 mm position DIC Slider Applicable Objective U DICT U DICTS Shift Type U DICTHC High Contrast Type U DICTHR High Resolution Type To be used in the BFP1 position of the DIC slider UIS2 Series UPlanSApo 10X2 IX2 DIC10 IX2 DIC10 20X IX2 DIC20 IX2 DIC20 IX2 DIC20HC IX2 DIC20HR 20XO 40X2 IX2 DIC40 IX2 DIC40 IX2 DIC40HC IX2 ...

Page 38: ...e provided instruction manual 2 Attaching the DIC Optical Elements Fig 44 The attaching method is identical to that for the phase contrast optical elements page 29 30 except that the positioning pin and positioning groove should be aligned when attaching each DIC optical element Align the positioning index 1 on the DIC prism with the positioning in dex 2 on a turret position and insert the DIC pri...

Page 39: ...nciples It is also possible to use the IX2 MDICT DIC mirror unit in place of the analyzer However the U MDICT3 that looks similar to the IX2 MDICT cannot be used because the analyzer s oscillation direction is different 1 Hold the IX2 AN analyzer 5 so that the side with indication faces up and insert the analyzer into the analyzer insertion slot 6 until it clicks 2 Hold the DIC slider so that the ...

Page 40: ...ular observation tube is used remove an eyepiece and attach the U CT30 centering telescope 5 Rotate the focusing ring 3 or with the centering telescope rotate the knurled section to bring the objective s exit pupil into focus Fig 50 6 Move the prism movement knob 7 of the DIC slider in the clockwise direction around the axis until the knob is stopped A black interference stripe then a rainbow colo...

Page 41: ...C U DICTHR With sensitive color observation using the U UCD8 engage the U UCDTP530 1 plate sensitive color plate in the light path Setting the background color to dark enables an observation like darkfield observation Setting the background color to gray provides observation with high con trast and 3D feeling with the gray sensitive color with which the sensitivity is highest Setting the backgroun...

Page 42: ...olarizer in the light path Fig 52 3 Attach the objective to be used to the revolving nosepiece and rotate it to engage the objective in the light path 4 Move the polarizer rotation clamping knob 2 on the polarizer unit hori zontally in the counterclockwise direction around the axis until the posi tion with which the field of view is darkest Fig 52 5 Clamp the polarizer rotation clamping knob so th...

Page 43: ... 2 Align index 2 on the IX2 SPT straight photo tube with positioning index 3 on the side port and fit the straight photo tube 3 Tighten the clamping screw 1 firmly 2 Attaching the Photo Eyepiece Fig 55 Use only a PE photo eyepiece for photomicrography Insert the PE photo eyepiece 1 into the photographic system mount on the straight photo tube The IX2 SPT or IX SPT is equipped with a plunger for us...

Page 44: ...ttached to the U BI90CT and U BI90 binocular observation tubes The finder eyepieces show four photo masks The numbers given to the masks correspond to the magnifications of the photo eyepiece Fig 57 1 Adjust the diopter of the eyepieces and focus on the photo masks in advance Focus so that the double crosslines are sharply visible as two distinct lines Fig 57 2 Bring the specimen into focus using ...

Page 45: ...ng points Reduce light intensity for observation as low as possible Use additional heat barrier filters for observation For time lapse photography synchronize the on off of the light bulb with the exposure 8 Setting the Filter and Lamp Intensity Set the lamp intensity and filters in accordance with the film to be used Film Filter Light Intensity Control Button Daylight color film 45LBD IF Camera s...

Page 46: ...dapter to be used 2 Attaching the Camera on the Trinocular Observation Tube H Fig 59 Compatibility with various mounts from different manufacturers can be obtained by attaching the IX SPT straight photo tube on the U TR30H 2 trinocular observation tube H and adding a TV adapter system for the BX series A C mount or F mount video camera cam be attached by attaching the U CMT C mount adapter or U FM...

Page 47: ...ttom panel Even after modification the binocular 100 observation or TV 100 ob servation are possible by setting the light path selector lever 5 TV Observation 1 Adjusting the microscope frame Turn on the light source and perform all the required adjustments for observation 2 Adjusting the video camera and monitor Perform the required adjustments such as the white balance and color adjustments The ...

Page 48: ...lving nose piece clicks properly into place Light path selector knob is set for the side port light path Set the knob to the binocular eyepiece light path position or increase the light intensity 13 Too many filters are used Reduce the number of filters to the minimum required 21 Stage center plate is engaged in the light path Move the stage and place the speci men again 17 Field iris diaphragm is...

Page 49: ... the field iris diaphragm image is formed in the specimen plane 23 e Image glares Condenser is raised too high Lower to the proper position 23 Aperture iris diaphragm is stopped down too far Open the aperture iris diaphragm 25 f Visibility of observed image is poor Image is not sharp Contrast is poor Details are poorly visible Objective in use is not designed for UIS2 UIS series Replace with an ob...

Page 50: ...ecimen holder 16 h Field of view of one eye does not match that of the other The interpupillary distance is incorrect Adjust the interpupillary distance 18 Incorrect diopter adjustment Adjust the diopter 18 You are not accustomed to parallel opti cal axis When looking into eyepieces do not stare at image from the beginning but see the overall field of view It is some times recommended to turn your...

Page 51: ...I90 Binocular tube with 45 tilting angle Diopter adjustment on one eyepiece possible Eye point switchable in 2 steps of 400 and 430 mm U TBI90 Binocular tube with 35 to 85 tilting angle Diopter adjustment on one eyepiece possible Eye point 406 to 471 mm U TR30H 2 Trinocular tube with 30 tilting angle Can be installed by means of the IX ATU intermediate tube Eye point 434 mm Condenser IX2 LWUCDA2 I...

Page 52: ... 1 3 WD 0 2 mm with correction collar UPlanFLN60X NA 0 9 WD 0 2 mm UPlanFLN60XOI NA 1 25 0 65 WD 0 12 mm with iris diaphragm UPlanFLN100XO2 NA 1 3 WD 0 2 mm UPlanFLN100XOI2 NA 1 3 0 6 WD 0 2 mm with iris diaphragm Plan semi apochromat for DIC and fluorescence observation LUCPlanFLN20X NA 0 45 WD 6 6 7 8 mm with correction collar LUCPlanFLN40X NA 0 6 WD 2 7 4 0 mm with correction collar LUCPlanFLN6...

Page 53: ... Specifications UIS Series For phase contrast observation SLCPlanFl40XPh NA 0 55 WD 7 7 mm ring slit IX PH2 with correction collar For DIC and brightfield observation SLCPlanFl40X NA 0 55 WD 7 7 mm with correction collar For reflected fluorescence Fura 2 observation UApo20X3 340 NA 0 75 WD 0 55 mm with oil proof cap UApo40X3 340 NA 0 9 WD 0 2 mm with oil proof cap UApo40XOI3 340 NA 1 35 WD 0 1 mm ...

Page 54: ...anuals Terasaki plate holder IX HOT Slide glass holder IX HOS Petri dish holder IX HOP For the connection of cables refer to the instruction manual for the IX2 UCB control box Mechanical stage IX MVR Stage Center plate Plain stage IX2 SP Cross stage IX SVL2 Cross stage IX2 SFR UCD adapter IX ADUCD Universal condenser U UCD8 Universal condenser IX2 LWUCD Motorized universal condenser IX2 LWUCDA2 Co...

Page 55: ... other switches than those listed below to the OFF positions S3 Set No 4 and No 5 to ON If the setting is not correct the objective may raise and hit the speci men during initialization Mounting the Z Board Fig 61 If other control boards than the U ZPCB T2 U ZPCB U AFA1 CB etc is installed in the IX2 UCB be sure to re move them before proceeding Leaving other control boards may result in malfuncti...

Page 56: ...t up the lamp housing 2 to remove it 3 Tilt the bulb socket 3 by 90 in the direction of the arrow 4 While pushing down the bulb clamping lever 4 down hold the halogen bulb 5 with gloves or a piece of gauze insert the bulb pins 6 straight and fully into the pin position 7 on the lamp socket Then return the lamp clamping lever gently back to the original position to clamp the bulb To prevent reduced...

Page 57: ...facing the front Fig 67 4 Screw the IX SCL stage clips 4 into the threaded holes provided on the stage top surface Fig 68 The IX2 SFR stage can be mounted by reversing the front and rear to implement a stage with knobs on the right position In this case however the left focusing knob comes in the way of the stage operation so it may be necessary to remove the left focusing knob by removing the cla...

Page 58: ...xis knobs into the U shaped notch on the positioning bracket 2 Using the Allen screwdriver tighten the clamping screw 3 of the bracket lightly to the stage 3 Align the stage s center alignment index line 4 with the edge 5 of the upper stage Fig 73 4 Slide the positioning bracket to and fro in order to determine the most convenient stalk angle 5 Tighten the bracket s clamping screw firmly Mechanica...

Page 59: ...oscopes Therefore if there are empty positions in the nosepiece attach the dust caps provided Fig 74 2 Attach the objective power indication stickers provided with the U HSTR2 hand switch to the hand switch according to the objective position num bers 6 Attaching the Condenser Figs 75 76 1 Loosen the condenser clamping screw 1 2 Fit the condenser into the mount dovetail on the condenser holder and...

Page 60: ... 78 2 Loosen the condenser clamping knob 3 Fig 78 3 Insert the mount of the UCD adapter into the mount dovetail of the con denser holder the condenser turret should be positioned on the right hand side Push the UCD adapter horizontally into the condenser holder until the condenser s positioning pin fits into the positioning groove on the mount dovetail 4 Tighten the condenser clamping knob 3 Fig 7...

Page 61: ...turned 180 and then attached on the observation tube mount again This lowers the eye point height by approximately 30 mm Fig 80 Trinocular Tube U TR30H 2 Figs 81 82 First mount the IX ATU intermediate tube 1 Using the Allen screwdriver loosen the observation tube clamping screw 1 on the observation tube mount Fig 81 2 Insert the circular dovetail mount of the intermediate tube into the obser vatio...

Page 62: ...e right in the figure Using the Accessory Mounting Holes Fig 84 Twelve threaded holes 1 are provided on the microscope frame for mount ing accessories such as a micromanipulator These holes are covered with plastic dust caps To remove a cap insert the tip of a flat blade screwdriver sideways into the space between the cap and the frame The applicable screws are M6 screws The screws can be inserted...

Page 63: ...t 1 Using the provided Allen wrench remove the three screws clamping the stabilizing feet from the bottom 2 Insert two of the rubber foot seats 1 into the rubber foot insertion sections on the rear of the microscope frame Perform Insertion in the rubber foot sections on the front as described above 3 Clamp the rear plate 2 directly without using the spacer Now clamp the microscope frame on the ant...

Page 64: ...g Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The fittings are to be marked with at least one of agencies listed in Table 1 In case you are unable to buy locally in your country the power supply cord which is approved by one of the agencies mentioned i...

Page 65: ...alter Österreichischer Verband für Elektrotechnik ÖVE Danmarks Elektriske Materialkontroll DEMKO National Standards Authority of Ireland NSAI Norges Elektriske Materiellkontroll NEMKO Asociacion Electrotecnica Y Electronica Espanola AEE Hellenic Organization for Standardization ELOT Instituto Portages da Qualidade IPQ Schweizerischer Elektro Technischer Verein SEV Elektriska Inspektoratet CEBEC HA...

Page 66: ...ems 1 More than 8 years have passed since original purchase or the total power ON time has exceeded 20 000 hours 2 Lamp does not light sometimes even though the main switch is set to on 3 Illumination flickers when you move the lamp cable or lamp housing 4 Lamp cable is unusually hot to the touch 5 Scorching or burning odor is produced during use 6 Illumination still flickers after replacement wit...

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Page 68: ...any 3500 Corporate Parkway P O Box 610 Center Valley PA 18034 0610 U S A One Corporate Drive Orangeburg NY 10962 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 31 Gilby Road Mount Waverley VIC 3149 Australia 5301 Blue Lagoon Drive Suite 290 Miami FL 33126 U S A EC REP 01 10 ...