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Condenser Holder
Condenser Lock Thumb Screw
d. Observe from the telescope. The bright ring
and dark ring should be coincided with each
other as shown in
Fig. 21 (d)
.
e. If the ring images are not clear, turn the top of
telescope until both ring images are in focus.
f. If the bright ring is still obscure as in
Fig. 21 (b)
,
adjust the condenser focusing knob.
g. If the two ring images are not coincided as
shown in
Fig. 21 (c)
, adjust the two centering
screws on the phase contrast condenser.
h. Remove the centering telescope and replace
the eyepiece.
8.3 Phase contrast kit of A1PHD:
1) Mounting the phase contrast kit
a. Replace the bright field objective(s) on nosepiece with
the phase contrast objective(s)
b. Thread the condenser ring plate onto the condenser as
shown in
Fig.22
.
c. Loosen the condenser lock thumb screw as shown in
Fig. 23
; take off the original condenser from the holder.
d. Insert the phase contrast condenser into the condenser
holder as shown in
Fig. 23
; tighten the condenser lock
thumb screw.
Note:
there are 3 phase contrast objectives: 10X, 40X and 100X, and there are 3
condenser ring plates: 10X, 40X and 100X. The corresponding objective and ring
plate must work together, i.e.
10X
phase contrast objective must work with
10X
condenser ring plate, and so on.
2) Centering the condenser ring plate
a. Connect the power cord to the microscope and insert the plug into a power
outlet.
b. Turn the desired phase contrast objective into light path.
c. Choose the ring plate that corresponding to the phase contrast objective in light
path and screw it on the condenser.
d. Replace the bright field condenser with the phase contrast condenser with ring
plate on.
(
a
) (
b
)
(
c
)
(
d
)
Fig. 21
Fig.23
Fig.22