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Condenser Holder
Condenser Lock Thumb Screw
Note:
There are 2 phase contrast objectives: 10X and 40X, and there are 2 condenser
annular rings on the sliding selector: 10/20 and 40. The corresponding objective,
annular ring and iris diagram must work together, i.e.,
40X
phase contrast
objective must work with
40
condenser annular ring and iris diagram
40
and so
on.
2) Centering the condenser ring plate
a. Connect the power cord to the microscope and
insert the plug into a power outlet.
b. Turn the desired phase contrast objective into
light path. Slide the sliding selector to the
corresponding annular ring and turn iris diagram
corresponding to the phase contrast objective in
the light path.
c. Remove one eyepiece from the microscope
eyepiece tube and insert the centering
telescope.
d. Observe from the telescope. The bright ring and
dark ring should be coincided with each other as
shown in
Fig. 21 (d)
.
e. If the ring images are not clear, turn the top of
telescope until both ring images are in focus.
f. If the bright ring is still obscure as in
Fig. 21 (b)
, adjust the condenser focusing
knob.
g. If the two ring images are not coincided as shown in
Fig. 21 (c)
, adjust the two
centering screws on the phase contrast condenser.
h. Remove the centering telescope and replace the eyepiece.
3) Performing the phase contrast observation
Perform the phase contrast observation the same way as a normal brightfield
microscope.
Note:
When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be
repeated following the procedures from 2)-b to 2)-h.
4) Performing the darkfield observation
a. Slide the sliding selector to the darkfield position, the condenser will work as a
darkfield condenser.
Fig.20
(
a
) (
b
)
(
c
)
(
d
)
Fig. 21
