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- 19 -
Fig. 22
Condenser Holder
Condenser Lock Thumb Screw
b. Turn iris diagram to 100.
c. Perform the darkfield observation the same way as a normal brightfield
microscope.
Note
:
The darkfield condenser works with the 4X, 10X, 40X regular brightfield
objectives.
The darkfield condenser does not work with the regular 100X
oil immersion objective.
The darkfield condenser works with the 100X oil darkfield
objective with iris diaphragm AJ100PR
(sold separately)
.
(optional)
When using the 100X oil darkfield objective, you will
need to drop the immersion oil on the top of the condenser &
on the top of the specimen, and adjust the iris ring (
Fig. 22
) to
get proper brightness and contrast of view field. The condenser
does not work well if no oil drop applied on the condenser.
(optional)
If air bubbles exist in the oil, clean the oil from the top of the condenser,
the top and bottom of slide with a lens cleaning paper.
(optional)
When you apply the immersion oil with the 100X objective, do not let
the immersion oil to contact with the dry objective lenses (especially the 40X). If
the immersion oil is on the dry objectives lens, please use the lens cleaning paper
to clean the objectives lens in time. The oil will damage the dry objective lenses.
(optional)
After observing the specimen with the 100X objective, use the lens
cleaning paper to gently clean the 100X objective lens, oil darkfield condenser
and the specimen in time.
(optional)
If it is hard to clean, you need a little bit alcohol to clean the 100X
objective lens, oil darkfield condenser and the specimen.
8.3 Phase contrast kit of A1PHD:
1) Mounting the phase contrast kit
a. Replace the bright field objective(s) on nosepiece with the
phase contrast objective(s)
b. Thread the condenser ring plate onto the condenser as
shown in
Fig.23
.
c. Loosen the condenser lock thumb screw as shown in
Fig. 24
;
take off the original condenser from the holder.
d. Insert the phase contrast condenser into the condenser holder as shown in
Fig.
24
; tighten the condenser lock thumb screw.
Iris
Ring
Fig.24
Fig.23