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Note:   

There are 3 phase contrast objectives: 10X, 40X and 100X, and there are 3 
condenser ring plates: 10X, 40X and 100X. The corresponding objective and ring 
plate must work together, i.e. 

10X

 phase contrast objective must work with 

10X

 

condenser ring plate, and so on. 

2)  Centering the condenser ring plate 

a. Connect the power cord to the microscope and insert the plug into a power outlet. 
b. Turn the desired phase contrast objective into light path. 
c.  Choose the ring plate that corresponding to the phase contrast objective in light 

path and screw it on the condenser. 

d. Replace the bright field condenser with the phase contrast condenser with ring 

plate on. 

e. Remove one eyepiece from the microscope eyepiece tube and insert the 

centering telescope.   

f.  Turn the light of microscope on and observe from the 

telescope.   

g. Turn the top of the telescope (

Fig.26

) until the dark 

ring image is in focus. If the dark ring is hard to find, 
put a normal white print paper on the stage and 
under the objective then you will see the dark ring as 
shown in 

Fig.25

 

(a)

.     

h. If the bright ring is still obscure as in 

Fig.25

 

(b)

, raise 

or lower the condenser by adjusting the condenser 
focusing knob (or the microscope focusing knob if 
necessary) till the bright ring is in focus and the dark 
ring is visible. 

i.  If the two ring images are not coincided as shown in 

Fig.25 (c)

, adjust the two centering screws on the 

condenser ring plate till the two rings are coincided 
with each other as shown in 

Fig.25 (d)

j.  Remove the centering telescope and replace it with the 

eyepiece. 

3)  Performing the phase contrast observation 

After you center the ring plate, you can perform the phase 
contrast observation the same way as a normal bright field 
microscope. 

Note:   

when change to another phase contrast objective and 
corresponding condenser ring plate, the focusing and 
centering of bright ring and dark ring should be repeated 
following the procedures from 

2)-b to 2)-h

Tips:   

 

Make the illumination as bright as possible. 

 

The thinner the specimen, the better the image.

 

 

(a) 

(b) 

(d) 

 

(c) 

Fig.25 

Fig.26 

Summary of Contents for MD827S30 series

Page 1: ...User Manual Digital Binocular Compound Microscope Model MD827S30 series MicroscopeNet com...

Page 2: ...1 Component Illustration 3 2 Installation 4 3 Operation 7 4 Specifications 10 5 Optional Parts 11 6 Troubleshooting Guide 13 7 Darkfield Condenser Installation and Operation Instructions 15 8 Phase C...

Page 3: ...cimen solutions or other liquids splash onto the stage objective or any other component disconnect the power cord immediately and wipe up the spillage Otherwise the instrument may be damaged 5 Importa...

Page 4: ...ng an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning more difficult Oil immersion objectives...

Page 5: ...ody 21 Condenser Focus Knob 4 Nosepiece 13 Focus Tension Ring 22 Focus Stop Lever 5 Objective 14 Coarse Focus Knob 23 Abbe Condenser 6 Slide Holder 15 Fine Focus Knob 24 Color Filter Holder 7 Mechanic...

Page 6: ...mechanical stage is at its lowest position 2 Turn the caps counter clockwise to remove them from the nosepiece 3 Take the objectives out from the plastic cases and turn each one clock wise into the h...

Page 7: ...You may also loosen the two screws on the cover to adjust the position of the bulb to get centered and even brightness See Fig 3 6 Screw the cover on Caution Before you turn over the microscope be su...

Page 8: ...he hole at the center of the black disc See Fig 5 You may try to get reflected ambient light on either side of the mirror with different angles for best result Note The mirror is only used when there...

Page 9: ...tance is correct when the left and right fields of view converge completely into one image 3 4 Adjusting eyepiece diopter 1 Rotate the 10x objective into position 2 Rotate the diopter rings on the eye...

Page 10: ...t When you use the 100X objective to observe the specimen you have to finish observing the specimen with the 4X 10X 40X objectives When you use the 100X objective to observe the specimen you have to a...

Page 11: ...into the USB port Fig 11 on the back of viewing head and the other end to the computer 3 Turn on the computer install the camera following the manual in the mini CD 4 Open image observing software to...

Page 12: ...ing knobs on both sides w focus stop Minimum fine focusing adjustment at 0 002mm range 28mm Mechanical Stage Double layer Dimension 5 1 2 x 5 1 2 140mmx140mm Translational range 3 x 2 75mm X 50mm Came...

Page 13: ...kfield Condenser Numerical Aperture Objective Mounting Size diameter A191 Dry 0 7 0 9 37mm A191BOIL Oil 1 36 1 25 Plan 100X 1 25 oil 0 5 160 0 17 spring w iris diaphragm 37mm 2 Carrying Case Model Dim...

Page 14: ...hase plate Achromatic 40X with built in phase plate spring Achromatic 100X with built in phase plate spring oil five positions 10 for 10X phase contrast objective 20 for 20X phase contrast objective 4...

Page 15: ...too thick or thin Use a slide cover of the appropriate thickness 0 17mm Slide may be upside down specimen at the bottom Turn slide over so the cover glass faces up Diopter adjustment is not set proper...

Page 16: ...e condenser is not in the right position Lower or raise the condenser slightly to the position that the specimen is the brightest A191BOIL only Make sure the oil contact the bottom of the slide and th...

Page 17: ...e condenser up or down slightly to get the best darkfield viewing Note The dry darkfield condenser is used with the dry objectives only The dry darkfield condenser works with the 4X 10X 40X objectives...

Page 18: ...Install the phase contrast objectives onto the nosepiece following the steps in 2 3 2 Mounting the phase contrast condenser annular ring disk a Rotate the nosepiece and set the 4x objective in positio...

Page 19: ...k being put at B position 6 Performing the phase contrast observation After centering the condenser ring plate perform the phase contrast observation the same way as a normal bright field microscope N...

Page 20: ...cope d Observe from the telescope The bright ring and dark ring should be coincided with each other as shown in Fig 21 d e If the ring images are not clear turn the top of telescope until both ring im...

Page 21: ...ptional When you apply the immersion oil with the 100X objective do not let the immersion oil to contact with the dry objective lenses especially the 40X If the immersion oil is on the dry objectives...

Page 22: ...is hard to find put a normal white print paper on the stage and under the objective then you will see the dark ring as shown in Fig 25 a h If the bright ring is still obscure as in Fig 25 b raise or l...

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