Page 9
4.1
Turn on the power supply using the power switch. Wait until the current display shows about
4.5 A. If the current falls below 4 A, replace the lamp. You should now wait for at least 10
minutes before aligning and using the lamp.
4.2
Aligning the mercury lamp
Move the filter selector of the filter wheel on the desired position, corresponding to an excitation
wavelength.
Insert a specimen slide and focus with 4X objective.
Fully open the field and aperture diaphgrams.
Adjust the two lamp centering screws on the case, in order to maximize the light intensity emitted by
the sample.
Adjust the focus of the collector lens until the sample is uniformly illuminated (if the lamp is not cor
-
rectly focussed, there will be a bright spot of light in the center of the field, and dark outside).
Note: if the current display shows 0 A for many seconds after switching on, press the Trigger button
(2-3 times if needed) until a current begins to flow in the lamp.
4.0 USING THE MICROSCOPE