22
A12.0910
According to the magnification of the objective used,
adjust the field diaphragm with the pole of fluorescence
pole. When the field diaphragm image is just at the
edge of field, the objective can provide best
performance and the image is the clearest.
(1) Rotate the turntable, to turn the fluorescence filter B
or G into the light path. (If there is no fluorescence
filter B or G, use another fluorescence filter.) (See Fig.
38)
(2) Rotate the objective nosepiece, to turn the 10X
objective into the light path.
(3) Push the fluorescence flashboard block
①
to the
position “O”, to open the light path.
(4) Focus the slide on the stage, and adjust it to be
clear.
(5) Pull the field diaphragm adjusting pole
③
to the
outmost to open the field diaphragm to the smallest,
while push it to the innermost to the largest.
(6) Observe through eyepiece to find image of field
diaphragm.
(7) Adjust two field diaphragm centering screws
②
on
the side of illuminator by a inner hexagon spanner, to
move the image to the center of view field.(See Fig. 38)
(8) Open the field diaphragm gradually. If the image of
field diaphragm is just inscribed to the view field, it
means the field diaphragm had been centered. (See Fig.
39)
(9) In actual use, please open the field diaphragm a
little to make it ex-scribed with view field in order to
obtain better image.
4-2-3 Center the Aperture Diaphragm
The aperture diaphragm decides the numerical aperture
of the illumination system. If the N.A. of illumination
system matches with the N.A. of the objective, it can
obtain better resolution and contrast, and increase the
depth of field.
Fig.39
Fig.38
