OPTO-EDU A12.0910, Instruction Manual

Page 1: ... guide troubleshooting and maintenance to the A12 0910 series biological microscope Please study this manual thoroughly before operating and keep it with the instrument The manufacturer reserves the rights to the modifications by technology development On the basis of operation ensured technical specifications may be subject to changes without notice ...

Page 2: ... Distance 12 3 7 Use the Eye cap 12 3 8 Adjust Stage 12 3 9 Center the Condenser 14 3 10 Adjust the Field Diaphragm 14 3 11 Adjust the Aperture Diaphragm 15 3 12 Use the Color Filter 15 3 13 Replace the Fuse 16 3 14 Assemble and Use the TV Device 16 4 Assembling and Operation of Fluorescence Illuminator 17 4 1 Assemblage Steps 17 4 2 Operation 21 5 Troubleshooting 26 5 1 Solutions of A12 0910 Seri...

Page 3: ...e range is supported as 100 240V Additional transformer is not necessary Make sure the voltage is in this range 8 Use the special wire supplied by our company 2 Maintenance 1 Wipe the lens gently with a soft tissue Carefully wipe off the oil marks and fingerprints on the lens surfaces with a tissue moistened with a small amount of 3 7 mixture of alcohol and ether or dimethylbenzene As the alcohol ...

Page 4: ...e Trinocular head Light Path Selecting Pole Inner Hexagon Spanner Body Voltage Indicator Light Source Group Reset Button For Intensity Mechanical Stage Condenser Illuminator Light Adjustment Knob Tension Adjustment Knob Switch Lever For Filters Y axis Moving Knob X axis Moving Knob ...

Page 5: ...2 A12 0910 Fine Focusing Knob Coarse Focusing Knob Condenser up down knob Dust Cover Coarse Adjusting Limit Ring Nosepiece Objective ...

Page 6: ...ents Trinocular head Flashboard knob XY Body Vertical centering Screw Blinkers Board Field Diaphragm Adjusting Pole Aperture Diaphragm Adjusting Pole Lens Focusing Knob Diaphragm Centering Screw Horizontal centering Screw Power Supply Box Vertical Fluorescence Illuminator ...

Page 7: ...4 A12 0910 ND Filter Slot ...

Page 8: ...ng Scheme and the numbers denote the assembling order Before assembling make sure there is no dust dirt or other matters to affect the assembly Assemble carefully and do not scrap any part or touch the glass surface 1 A12 0910 Series Biological Microscope Assembling Scheme ...

Page 9: ...6 A12 0910 2 A12 0910 Series Biological Fluorescence Microscope Assembling Scheme ...

Page 10: ...t on the bulb please wipe it with clean soft cloth Bulb selected only 12V 100W Halogen bulb Philips 7724 Before replacing the bulb make sure to cut off the main power and wait for both the bulb base and bulb cooling down 2 2 2 Assemble the Light Source Group Push the light source holder into the body holder Make the light source group to be horizontal with the body group and lock the screw See Fig...

Page 11: ...Fig 6 2 Match the dovetail interface of the nosepiece with the dovetail groove of the arm and push it to the bottom Tighten the lock screw 2 2 6 Assemble the Head 1 Loosen the head lock screw fully See Fig 7 2 From a little right position insert the coattail interface on the bottom of head into the hole of middle head with a little left inclined Keep the two eyepiece tubes forward and then screw d...

Page 12: ...the bottom 2 2 9 Connect Power Cord 1 Make sure the main switch is at O OFF position 2 Match the gap of the lower light source aviation plug to the gap of the aviation socket and insert it to the bottom See Fig 10 3 Insert one end of power cord into the power socket of the microscope 4 Insert the other end of power cord into the power supply socket Don t use strong force when the power cord is ben...

Page 13: ...y Rotate it in clockwise to raise the light intensity while lower it in counterclockwise The number on the right on Voltage Indicator show the voltage Use of bulbs in the low voltage state can extend the bulb life When the light intensity reset button is pressed rotate the light adjustment knob does not work The light intensity of this machine is preseted to the best for photomicrography by LBD fi...

Page 14: ... 1 Put the slice on the stage and hold it down with the clip Shift the 4X objective into the light path See Fig 14 2 Loosen the random upper limit knob then observe the right eyepiece with the right eye Rotate the coarse focusing knob until the image appears in the view field then lock the random upper limit knob The random upper limit knob can prevent the objective touching the slice when focusin...

Page 15: ...cale of the interpupillary distance indicator The scale value is the interpupillary distance Adjustable range 50 76mm Remember your eye s interpupillary distance so that you can use it next time 3 7 Use the Eye cap 1 Turn over the eye cap if the user is wearing glasses so that it can prevent the glasses touching the eyepieces and avoid damaging to both glasses and eyepieces 2 Open the eye cap if t...

Page 16: ...ample holder and move left and right the stage rail hit the limit stopper Vertical Hold the top surface of the stage and move back and forth hit the limit stopper For reflected illumination Lower the stage bracket the microscope can adapt the height of sample no more than 35mm which is very useful when observing metallurgical samples and other thick objects 1 Move the stage to the lowest and then ...

Page 17: ...ing screw to put the image to the center of the field of view 7 Open the field diaphragm gradually If the image is in the center all the time and inscribed to the field of view it shows condenser has been centered correctly See Fig 22 8 In actual use you can enlarge the field diaphragm a bit and make the image circumscribed to the field of view 3 10 Adjust the Field Diaphragm By limiting the diame...

Page 18: ...the 80 value of objective N A see Fig 24 In actual use adjust the aperture diaphragm according to the size of the sample image contrast until it s comfortable for observation and the contrast is good 3 12 Use the Color Filter The color filter can make the background light more suitable and strengthen the image contrast When an external color filter is used put a diameter 45mm filter into the groov...

Page 19: ...from the above flute and replace it with a new one Then put it to the flute and push the fuse holder into the socket until a sound of kada is heard See Fig 26 Specifications of the fuse 250V 3 15A 3 14 Assemble and Use the TV Device 1 Loosen the lock screw of trinocular head and take out the dust cover See Fig 27 2 Take down the dust cover of the TV adapter Insert the TV adapter into the trinocula...

Page 20: ...2 The blinker board is installed in the fluorescence filter group When using the fluorescence filter group first loose the lock screw with the inner hexagon spanner and take off the blinker board See Fig 30 3 Put the diaphragm slice of the fluorescence filter group which is to be assembled upward and match the dovetail groove of the fluorescence filter group with the dovetail wedge of the front co...

Page 21: ...supporting rod and then insert the positive side big end of the new mercury lamp into the positive holder thoroughly then put the negative holder on the negative side small end of bulb and lock the screw See Fig 33 Please make sure the mercury lamp is put vertically If there is aspirating hole on bulb please make sure the hole directly to the ceramic holder 3 Put the bulb holder into bulb house an...

Page 22: ...l interface on the bottom of head into the hole of middle head with a little left inclined Keep the two eyepiece tubes forward and then screw down the lock screw 4 1 8 Assemble the Eyepiece and the Objective 1 Assemble the objective according to 2 2 7 Assemble the Objective 2 Assemble the eyepiece according to 2 2 8 Assemble the Eyepiece 4 1 9 Connect Power Cord 1 Make sure the main switch of micr...

Page 23: ... strong force when the power cord is bended or twisted otherwise it will be damaged Use the special wire supplied by our company which can only be used for power supply for mercury lamp box Connect the power cord appropriately to make sure the instrument is connected to ground ...

Page 24: ...and seconds will clear 4 The indicating range of the current CURRENT is 0 9 99A No need to turn on the main switch when using fluorescence illumination To avoid damage do not cut off power supply within 15 minutes after mercury lamp light on In order to prolong the life of mercury lamp and the power supply box please do not re light on it within 3 minutes after turned off When the timer indicates ...

Page 25: ...o the outmost to open the field diaphragm to the smallest while push it to the innermost to the largest 6 Observe through eyepiece to find image of field diaphragm 7 Adjust two field diaphragm centering screws on the side of illuminator by a inner hexagon spanner to move the image to the center of view field See Fig 38 8 Open the field diaphragm gradually If the image of field diaphragm is just in...

Page 26: ...of aperture diaphragm in the view field 7 Adjust the two aperture diaphragm centering screws on the side of illuminator by a inner hexagon spanner to move the image to the center of view field See Fig 40 8 Open the aperture diaphragm gradually if the image is inscribed with the view field then it means the aperture diaphragm is rightly centered 9 In actual fluorescence observation push the apertur...

Page 27: ...ing lens and the centering screw to make the reflected image projected on the scale of the centering objective Fig 41 42b 6 Adjust the centering screw to make the filament image and reflected image symmetry to the scale of the centering objective Adjust the focusing screw to make both images with same size Fig 41 42c 7 Adjust the vertical adjusting screw of mercury lamp and the horizontal adjustin...

Page 28: ...2 Fasten the flute under the fuse holder by fingers take out the fuse holder from socket Then take off the fuse from the above flute and replace it with a new one Then put it to the flute and push the fuse holder into the socket until a sound of kada is heard See Fig 43 4 2 6 Install the Filter The color filter can make the background light more suitable and strengthen the image contrast According...

Page 29: ...in or dust is observed in the field of view Stains have accumulated on the specimen Clean the specimen Stains have accumulated on the lens Clean the lens 4 Unclear image No cover glass on the specimen slide Add the cover glass The cover glass is not standard Use a standard cover glass with thickness of δ0 17mm The specimen faces down Put the specimen to face up The immersion oil has accumulated on...

Page 30: ...ion adjustment knob is too loose Tighten it to an appropriate position 11 Coarse focusing knob cannot rise The coarse focusing limit knob is locked Loosen the coarse focusing limit knob 12 Coarse focusing knob cannot decline The condenser is too low Raise the condenser 13 Cannot move the slide smoothly The slide is not fixed correctly Adjust it correctly The movable specimen holder is not fixed pr...

Page 31: ...he filter block Use wrong immersion oil Use a correct one 3 Blur field or brightness asymmetry Nosepiece is not at lock position Turn the nosepiece until a sound of kada is heard to lock position Filter blocks are not at correct position Adjust them Mercury lamp filament is not centered Center it Condenser adjusting knob is not at correct position Adjust it 4 Mercury lamp does not light No power s...