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1.
Applications
Some objects can emit a ray which wavelength is longer than that of the
excitation light when irradiated. This ray is called fluorescence, and observers
can study the objectives through fluorescence microscope using the
phenomenon.
Fluorescence microscope has wide applications in basic theory research and
clinical diagnosis about medicine, biology, as well as analysis and test in
industry, agriculture, stockbreeding, criminal investigation, legal medical
appraise, environmental protection etc.
2.
Principle
The device consisting of
Epi-fluorescence
illuminator, 100W direct
current mercury lamp
power box, fluorescence
objectives is combined
with
microscope
to
make up fluorescence
microscope. The device
is
designed
and
manufactured
with
Epi-excitation principle
and standard provided
with
four
group
excitation filters system:
blue (B), green (G),
violet (V) and ultraviolet
(UV).
The light emitted from the lamp is converted to the excitation light (e.g.blue
light) with specified wavelength by going through the excitation filter, then
passes through dichroic prism and objectives (the objective plays role of
condenser) to irradiate vertically the object. The object is excitated and emits
fluorescence with specified wavelength (e.g. green and yellow) and make
image passing through objectives, dichroic prism and eyepieces. The light
(including excitation light) without fluorescence wavelength is reflected or
absorbed by dichroic prism and barrier filter, and can not reach the view
system. Therefore, what can be seen in the view field is the bright
fluorescence image against the dark background.
Fluorescence Device Diagram
Fluorescence
module
Condenser
prism
Mercury
lamp
tube
Barrier filter
Dichroic
Excitation filter
Eyepiece
Objective
Specimen
