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© 2003, Pall Corporation. Pall,

, Omega, and Minimate are trademarks of Pall Corporation. ® indicates a trademark

registered in the USA.                                                             is a service mark of Pall Corporation. All rights reserved.

*PharMed Masterflex is a registered trademark 6485 of Norton who makes tubing, DuPont makes material.

4/03, Xk, GN03.0678

PN88227

Pall LIfe Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA

800.521.1520  toll free in USA
734.665.0651  phone 
734.913.6114  fax

Pall Corporation
2200 Northern Boulevard
East Hills, NY 11548 USA

800.717.7255  toll free in USA
516.454.4500  phone 
516.625.3610  fax

Visit us on the Web at www.pall.com/lab

E-mail us at Lab@pall.com

Australia – Lane Cove, NSW
Tel: 02 9428-2333
1800 635-082 (in Australia)
Fax: 02 9428-5610
Austria – Wien
Tel: 043-1-49 192-0
Fax: 0043-1-49 192-400
Canada – Ontario
Tel: 905-542-0330
800-263-5910 (in Canada)
Fax: 905-542-0331
Canada – Québec
Tel: 514-332-7255
800-435-6268 (in Canada)
Fax: 514-332-0996
800-808-6268 (in Canada)
China – P. R., Beijing
Tel: 86-10-8458 4010
Fax: 86-10-8458 4001
France – St. Germain-en-Laye
Tel: 01 30 61 39 92
Fax: 01 30 61 58 01
Lab-FR@pall.com

Germany – Dreieich
Tel: 06103-307 333
Fax: 06103-307 399
Lab-DE@pall.com
India – Mumbai
Tel: 91-22-5956050
Fax: 91-22-5956051
Italy – Milano
Tel: 02-47796-1
Fax: 02-47796-394

or 02-41-22-985

Japan – Tokyo 
Tel: 3-3495-8319
Fax: 3-3495-5397
Korea – Seoul
Tel: 2-569-9161
Fax: 2-569-9092
Poland – Warszawa
Tel/Fax: 22-835 83 83
Russia – Moscow
Tel: 095 787-76-14
Fax: 095 787-76-15

Singapore
Tel: (65) 389-6500
Fax: (65) 389-6501
Spain – Madrid
Tel: 91-657-9876
Fax: 91-657-9836
Sweden – Lund
Tel: +46 (0)46 158400
Fax: +46 (0)46 320781
Switzerland – Basel
Tel: 061-638 39 00
Fax: 061-638 39 40
Taiwan – Taipei
Tel: 2-2545-5991
Fax: 2-2545-5990
United Kingdom – 
Portsmouth
Tel: 023 92 302600
Fax: 023 92 302601
Lab-UK@pall.com

18

Product No.

Description

OAD65C12

Minimate TFF Capsule w/650 dalton Omega

OA001C12

Minimate TFF Capsule w/1Kd Omega

OA003C12

Minimate TFF Capsule w/3Kd Omega

OA005C12

Minimate TFF Capsule w/5Kd Omega

OA010C12

Minimate TFF Capsule w/10Kd Omega

OA030C12

Minimate TFF Capsule w/30Kd Omega

OA050C12

Minimate TFF Capsule w/50Kd Omega

OA070C12

Minimate TFF Capsule w/70Kd Omega

Ordering Information

Minimate TFF capsules; Self contained Ultrafiltration Tangential Flow Filtration capsule with Luer Connectors

Product No.

Description

OA100C12

Minimate TFF Capsule w/ 100Kd Omega

OA300C12

Minimate TFF Capsule w/ 300Kd Omega

OA500C12

Minimate TFF Capsule w/ 500Kd Omega

OA990C12

Minimate TFF Capsule w/ 1000Kd Omega

88216

Minimate Fittings kit, Contains: Male luer to 
3.2 mm (1/8") hose barb, Female luer to 
3.2 mm(1/8") hose barb, 3.2 mm (1/8") i.d.
tubing, Tubing screw clamp, Tubing clamps,
Adhesive strips (loop and hook)

Product No.

Description

FS700X06

3-port valves (6)

FS700M01

MINIM™ System Pump station with 500mL
reservoir, 1.6mm (1/16 in.) i.d. tubing,
3.2 mm (1/8 in.) i.d. tubing, 3 port valves, 
fittings. Multi-voltage unit. Supplied with 
detachable power cord.

FS720M01A

Pressure Display Unit*
Display reading in psi,100-120VAC, 60 Hz

FS720M01B

Pressure Display Unit*
Display reading in bar,200-240VAC, 50 Hz

*Three digital displays for Feed, Retentate and Filtrate
Pressure (includes 6 pressure transducers).

Complementary Products

Product No.

Description

FS700X14

Pressure Transducer (1), Sterile Packed (for 
use with PN FS720M01A and FS720M01B)

FS700X15

Pressure Transducers (12)
Individually Sterile Packed (for use with 
PN FS720M01A and FS720M01B)

FS710M01

Pressure Gauge Assembly (1), (two or three 
recommended: feed, retentate, and filtrate)

FS007X75

500 mL Ultrareservoir, includes pressure 
gauge, fittings and three-way valve

Summary of Contents for Minimate OA001C12

Page 1: ... Buffer Conditioning Optional 5 Concentration Diafiltration 6 Product Recovery 6 Cleaning 6 Storage of the Minimate TFF Capsules 6 Disposal of the Minimate TFF Capsule 6 Appendix A 7 Normalized Water Permeability 7 System Hold up Volume 10 Minimate TFF Capsule Integrity Test 10 Flushing and Cleaning After Use 12 Appendix B 14 Specifications 14 Certification 15 Ordering Information 15 Support Liter...

Page 2: ...tration Replace dialysis applications Depyrogenate water buffers and media solutions Product Information The Minimate TFF Capsule comes with threaded luer plugs in each of the 4 ports A fitting package with the following components is included Fittings Package Part No 88216 includes A Tubing 3 2 mm 1 8 i d 120cm 4 ft B Male luer to hose barb fittings 3 2 mm 1 8 2 C Female luer to hose barb fitting...

Page 3: ...ing to one of the filtrate vent ports Note Depending on the orientation of the capsule choose the filtrate port that is at the highest elevation This allows air to be completely expelled from the filtrate side of the membrane The filtrate channel can be drained easily by opening the other filtrate port as a vent 10 Attach a piece of tubing to the filtrate hose barb 11 Install a tubing clamp over e...

Page 4: ...mL min Slowly tighten retentate screw clamp to increase backpressure This will increase flow through the filtrate lines Tighten clamp until filtrate flow rate is approximately equal to the retentate flow rate or until feed pressure reaches 2 bar 30 psi Do not exceed a feed pressure of 2 bar 30 psi Pump at least 250 mL of the water through the retentate tubing and 250 mL through the filtrate tubing...

Page 5: ... Test Optional An integrity test is performed to identify any gross defects in the device membrane or seals that could cause product loss prior to use Capsules are 100 integrity tested after manufacturing Therefore it is not necessary to perform an integrity test on a new Minimate TFF Capsule However for critical applications or if the capsule will be reused it is recommended that an integrity tes...

Page 6: ...inutes to try and recover additional product The remaining liquid in the system can be pumped out into a separate container by allowing air to be pumped through capsule to displace it A decision can then be made whether to combine this volume with the main product Product in Filtrate If the product is in the filtrate raise the vent port so it is higher than the collection vessel Make sure the end ...

Page 7: ... is shown below To determine the NWP a pressure gauge is required on the feed and retentate ports Filtrate pressure should be zero 0 provided there are no restrictions on the filtrate line and that the filtrate line is open to atmosphere Recirculate the filtrate and retentate streams back to the feed reservoir to minimize the volume of water needed Removing air from the retentate All air must be r...

Page 8: ...Rate LMH Filtrate Flux Rate mL min cm2 x 600 Water Permeability LMH TMP Filtrate Flux Rate LMH TMP NWP LMH TMP 20 C Filtrate Flux Rate LMH x Temperature Correction Factor TCF at operating temperature Table 3 Temperature Correction Factors TCF 20 C for determining Normalizing Water Permeability Temperature Correction Factors TCF 20 C for determining Normalizing Water Permeability T C TCF 20 C T C T...

Page 9: ... 15 Filtrate Pressure psi bar 0 0 0 TMP psi bar 5 10 15 Filtrate Flow Rate mL min 4 6 9 2 13 8 Filtrate Flux Rate mL min cm2 0 092 0 184 0 276 Filtrate Flux Rate LMH 55 110 165 Water Permeability LMH TMP 11 NWP LMH TMP 20 C 12 2 Water Permeability 110 LMH 10 psig 11 0 LMH psig Normalized Water Permeability 11 0 LMH psig x TCF 20 C where TCF 20 C 1 109 Correcting water permeability for temperature ...

Page 10: ... to determine Minimum Working Volume 1 Add water to the reservoir 2 Direct retentate and filtrate lines into feed reservoir Open the retentate clamp and close the filtrate clamp 3 Adjust pump to deliver the operating retentate flow rate that will be used for the process 4 Direct the retentate line to drain Watch the water in the reservoir When the water reaches a level where it appears that air is...

Page 11: ...Integrity Test Procedure 1 Empty the feed reservoir 2 Open retentate and filtrate screw clamps and pump out liquid from feed and retentate tubing 3 Fill a 400 mL or larger beaker and a 25 mL graduated cylinder with water 4 Invert the full graduated cylinder into the large beaker Place a piece of paper or Parafilm over the mouth of the cylinder Turn the cylinder over and remove the piece of paper a...

Page 12: ... C 0 1N nitric acetic or phosphoric acid Post Treatment of Minimate TFF Capsule Before introducing the cleaning solution into the membrane system remaining product and contaminants should first be flushed free using either buffer or spent filtrate The use of a buffered flush will eliminate possible solubility changes Spent filtrate assuming the product is in concentrate eliminates the need for fre...

Page 13: ...recondi tioned described on page 5 Water should be wateWFI or at a minimum 0 2 µm filtered DI water All water permeability rates are normalized to a temperature of 20 C by using a temperature correction factor TCF 20 C in Table 3 Determining the water permeability after cleaning 1 Add water to the reservoir 2 Return the retentate and filtrate line back to the feed reservoir 3 Open the retentate an...

Page 14: ...cm x 3 8cm x 1 8cm L x W x H nominal 8in x 1 5 in x 0 7 in Effective Filtration Area 50 cm2 Product Hold Up Volume feed retentate 1 6mL Forward Flow Air Integrity Value 7 mL min at 0 75 bar 10 psi Operating Temperature Range 5 ºC 41 ºF to 50 ºC 122 ºF Maximum Operating Pressure at 20 ºC 68 ºF 4 bar 400 kPa 60 psi Recommended Crossflow 30 40 mL min 0 6 0 8 Liters min ft2 Connections Feed Female Lue...

Page 15: ...cedures PN88227 is supplied on the Minimate TFF Capsule CD Rom PN88201 enclosed with the product Many additional accessories are available for this product including pumps reservoirs and connectors For further assistance please consult with your local Pall representative or distributor Support Literature Minimate TFF Capsule Data Sheet USD 2218 Minimate TFF Capsule Quick Start Guide PN 88200 Minim...

Page 16: ...ure Readings Flow Rate Flux Rate Volume Conc Diafiltrate Comments Time Temp Feed Retent Filtrate TMP Delta P Retent CFF Filtrate Filtrate Feed Filtrate Factor Volumes hr min C psi bar psi bar psi bar psi bar psi bar L min L min ft 2 mL min LMH mL mL x DV Initial Water Data Filtrate Flux Rate Delta P Process Data Optimization Yes or No Final Water Data Filtrate Flux Rate Delta P ...

Page 17: ... Measured filtrate flow rate Filtrate Flux Rate LMH Filtrate flow rate membrane area Liters hour meter2 Volume Feed mL Remaining Feed Volume Starting Volume Total collected filtrate volume Starting Volume equals process volume added to reservoir plus hold up volume if system was filled with liquid Filtrate mL Total collected filtrate volume Concentration Factor X Starting Volume Starting Volume Fi...

Page 18: ...2 2545 5990 United Kingdom Portsmouth Tel 023 92 302600 Fax 023 92 302601 Lab UK pall com 18 Product No Description OAD65C12 Minimate TFF Capsule w 650 dalton Omega OA001C12 Minimate TFF Capsule w 1Kd Omega OA003C12 Minimate TFF Capsule w 3Kd Omega OA005C12 Minimate TFF Capsule w 5Kd Omega OA010C12 Minimate TFF Capsule w 10Kd Omega OA030C12 Minimate TFF Capsule w 30Kd Omega OA050C12 Minimate TFF C...

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