QIAcuity User Manual 06/2022
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QIAcuity Nanoplate 8.5K 96-well
In this plate, one reaction mix is distributed in 1 well and separated into approximately 8500
partitions. This plate is recommended for applications using low input volumes and large number
of samples. The plate may be used for up to 96 samples and has a grey frame as distinction from
the other plates.
The key applications of this nanoplate are:
CNV detection
NGS library quantification
Reaction setup
Note
: The QIAcuity reads fluorescence from the bottom of the plate, which is covered with a foil.
For best results, keep the foil clean and avoid damages such as scratches. Also, keep the barcode
on the side of the plate clean and intact. Ensure that you wear gloves when working with a plate
and do not apply force to it.
For better handling of the plate, you can place the plate into the Nanoplate tray that can be ordered
as accessory, see Appendix B – QIAcuity Accessories or the QIAcuity webpage on
www.qiagen.com
.
To set up a plate, follow these steps:
1.
Prepare your master mix according to your reaction setup. To prepare the reaction mix
without sample, the QIAcuity PCR Master Mix has to be mixed with primers, RNase-free water
and optionally restrictions enzyme and probes according to the kit manual. The final volume
depends on the QIAcuity Nanoplate which is used (refer to Table 19).
Note
: To prevent non-homogeneous reaction mixes, the set up in a standard PCR pre-plate is
required. The calculated reagent volumes have to be pipetted into the PCR pre-plate, and then
the sample has to be added accordingly. For homogenous mixing of reaction mix, the pre-
plate has to be sealed, shortly vortexed and briefly centrifuged.
Note
: Enzymatic fragmentation of DNA larger than 20 kilobase ensures even distribution of
template throughout the QIAcuity Nanoplate, which in turn leads to accurate and precise
quantification. Therefore, adding a restriction enzyme depends on the template used. In case
of enzymatic fragmentation using the recommended restriction enzymes, the pre-plate has to
be incubated at RT for 10 minutes. Longer incubation doesn’t lead to unspecific restriction and
Summary of Contents for QIAcuity 911000
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