TissueLyser II User Manual 05/2016
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General Description
The TissueLyser II is a flexible bead mill designed for rapid and efficient disruption of up to 48 or
192 samples at the same time. A wide variety of biological samples can be processed, including
human, animal, and plant tissues as well as yeast and bacteria. Each sample is simultaneously
disrupted and homogenized by high-speed shaking with a bead in a sealed tube (bead milling).
TissueLyser II principle
Efficient sample disruption is a prerequisite for nucleic acid and protein purification procedures.
Incomplete sample disruption can lead to significantly reduced yields and can increase the risk of
clogging when using purification columns or magnetic particles. The TissueLyser II thoroughly
disrupts and simultaneously homogenizes biological samples in the presence of lysis buffer. Plant
tissues can alternatively be disrupted and homogenized in the absence of lysis buffer.
Tubes containing sample and bead are placed in a TissueLyser Adapter Set and shaken at a very
high speed, causing the samples to be disrupted and homogenized by the beating and grinding
effect of the beads. The tubes can be discarded after use, which prevents cross-contamination and
provides time savings, as there is no need to clean tubes after disrupting samples. In addition,
there is no cross-contamination during sample disruption as each tube is securely sealed with its
own lid.
The TissueLyser II can also disrupt and homogenize large samples when used in combination with
a Grinding Jar Set. Samples are placed in a 10 ml jar containing a grinding ball and shaken at
high speed.
TissueLyser Adapter Sets, Grinding Jar Sets, and sample tubes can be precooled to minimize
degradation of nucleic acids during sample disruption. To find out which procedures require
precooling, see the
TissueLyser Handbook
.
