Qiagen TissueLyser II, User Manual

Page 1: ...Sample to Insight__ May 2016 TissueLyser II User Manual For disruption of up to 48 or 192 biological samples...

Page 2: ...About this user manual 15 Intended use of the TissueLyser II 15 Damage to the clamps of the TissueLyser II 16 Requirements for TissueLyser II users 16 General Description 17 TissueLyser II principle...

Page 3: ...30 Operating Procedures 32 Sample disruption 32 Storing disruption parameters 33 Displaying the accumulated operating time 34 Displaying the software version 34 Maintenance Procedures 35 Servicing 35...

Page 4: ...TissueLyser II User Manual 05 2016 4 Appendix B 44 Liability clause 44 Notes 45 Notes 46...

Page 5: ...his manual is intended to supplement not supersede the normal safety requirements prevailing in the user s country Proper use WARNING Risk of personal injury and material damage W1 Improper use of the...

Page 6: ...sconnection of the protective conductor terminal is likely to make the TissueLyser II dangerous Intentional interruption is prohibited Lethal voltages inside the TissueLyser II When the TissueLyser II...

Page 7: ...the instrument C3 Direct sunlight may bleach parts of the instrument and cause damage to plastic parts The TissueLyser II must be located out of direct sunlight Biological safety Specimens and reagen...

Page 8: ...yser II may be hazardous or may become hazardous after completion of the sample disruption process Always wear safety glasses gloves and a lab coat The responsible body e g laboratory manager must tak...

Page 9: ...can occur if the TissueLyser Adapter Set or Grinding Jar Set is not securely attached Ensure that both adapters or grinding jars are of similar weight Do not operate the TissueLyser II with only a si...

Page 10: ...sch digung des Ger ts f hren Der TissueLyser II darf auf keinen Fall w hrend des Betriebs bewegt werden Die Bedienung des TissueLyser II darf nur durch qualifiziertes entsprechend geschultes Personal...

Page 11: ...te interruption du conducteur de protection conducteur de terre de masse l int rieur ou l ext rieur du TissueLyser II ou toute d connexion de la borne du conducteur de protection est susceptible de re...

Page 12: ...orleiter muss alle erforderlichen Vorsichtsma nahmen treffen um sicherzustellen dass die unmittelbare Umgebung des Arbeitsplatzes sicher ist und die Bediener des Ger ts ausreichend geschult sind Au er...

Page 13: ...festgelegt sind nicht berschritten werden Beim Betrieb eines Abzugs und bei der Entsorgung von Abfallstoffen m ssen alle Bestimmungen und Gesetze zu Gesundheitsschutz und Sicherheit am Arbeitsplatz a...

Page 14: ...ached Ensure that both adapters or grinding jars are of similar weight Do not operate the TissueLyser II with only a single adapter or grinding jar installed DE Sich bewegende Ger teteile Wenn das Tis...

Page 15: ...3 General Description 4 Installation Procedures 5 Operating Procedures 6 Maintenance Procedures 7 Troubleshooting Guide Appendices The appendices contain the following Technical data Warranty terms I...

Page 16: ...er II without straining the clamping mechanism Damage to the clamps of the TissueLyser II will occur if other components of reduced thickness are installed Therefore the TissueLyser II must not be use...

Page 17: ...resence of lysis buffer Plant tissues can alternatively be disrupted and homogenized in the absence of lysis buffer Tubes containing sample and bead are placed in a TissueLyser Adapter Set and shaken...

Page 18: ...at the power switch located at the back of the instrument Oscillation frequency can be adjusted in steps of 0 1 Hz using a keypad Figure 3 located on top of the instrument Disruption time from 10 sec...

Page 19: ...TissueLyser II User Manual 05 2016 19 Figure 2 TissueLyser II open The transparent hood is raised and a TissueLyser Adapter Set is installed...

Page 20: ...of 48 samples in parallel using standard 2 ml microcentrifuge tubes e g Eppendorf Safe Lock micro test tubes The adapter set contains 2 adapters with each adapter comprised of a top plate a bottom pla...

Page 21: ...560 and 19566 The adapter set contains 2 adapters with each adapter comprised of a top plate and a bottom plate for holding a rack of capped collection microtubes Figure 5 Sample disruption can be car...

Page 22: ...rogen For more information see the product sheet supplied with the Grinding Jar Set Figure 6 Grinding Jar Set S Steel Each 10 ml jar is made of stainless steel and contains a 20 mm diameter stainless...

Page 23: ...Bead Dispenser 5 mm cat no 69965 Dispenses beads of 5 mm diameter and contains a reservoir that can hold approximately 150 beads TissueLyser Single Bead Dispenser 7 mm cat no 69967 Dispenses beads of...

Page 24: ...m diameter and contains a reservoir that can hold approximately 1000 beads TissueLyser 5 mm Bead Dispenser 96 Well cat no 69975 Dispenses beads of 5 mm diameter and contains a reservoir that can hold...

Page 25: ...Steel Beads 7 mm cat no 69990 Tungsten Carbide Beads 3 mm cat no 69997 Applications The TissueLyser II enables disruption and homogenization of Human and animal tissues Human and animal cells Plant t...

Page 26: ...TissueLyser II User Manual TissueLyser Handbook If anything is missing contact QIAGEN Technical Services Check that the TissueLyser II is not damaged If anything is damaged contact QIAGEN Technical Se...

Page 27: ...ity device installed can lead to damage to the mechanical components of the instrument Site requirements Place the TissueLyser II on a flat stable surface and ensure that there is sufficient space aro...

Page 28: ...TissueLyser II with both hands below the base of the instrument Please retain all original packaging for the duration of the warranty period and beyond A charge may be incurred in the event of damage...

Page 29: ...Set to the TissueLyser II as follows Pull the locking pin A upward out of its slot and rotate it by 90 Figure 11 This releases the locking device Turn the handwheel B counterclockwise until the maximu...

Page 30: ...ed in this user manual may lead to damage to the clamps of the TissueLyser II WARNING Moving parts Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is not securel...

Page 31: ...5 2016 31 Figure 12 Backside of TissueLyser II A Power switch B Fuse compartment C Power cord socket D RS 232 port for service personnel E Safety information plate F Voltage rating plate G Safety info...

Page 32: ...te the TissueLyser II with only a single adapter or grinding jar installed 2 Make sure that the TissueLyser II is connected to a power outlet 3 Switch on the TissueLyser II using the power switch 4 Ch...

Page 33: ...key on the keypad To continue operation of the TissueLyser II from the time remaining press the START key To cancel operation of the TissueLyser II press the STOP key twice The TissueLyser II then goe...

Page 34: ...in standby mode simultaneously press the SET key and the key under the Frequency 1 s display 2 bS appears in the Memory display In addition the accumulated operating time for the TissueLyser II is dis...

Page 35: ...I is supplied with a warranty that lasts for 12 months starting from the date of shipment The warranty includes all repairs due to mechanical breakdown Regular maintenance The TissueLyser II should be...

Page 36: ...opanol per 100 g liquid or Mikrozid Liquid Sch lke Mayr GmbH cat no 109160 Disinfectants based on glyoxal and quaternary ammonium salt can be used for submerging the TissueLyser Adapter Sets and Grind...

Page 37: ...ssueLyser Adapter Sets and Grinding Jar Sets WARNING Toxic fumes Do not use bleach to clean or disinfect used labware Bleach in contact with salts from the buffers used can produce toxic fumes CAUTION...

Page 38: ...h itself on again If there is a defect contact QIAGEN Technical Services to request repair of the instrument TissueLyser Adapter Sets and Grinding Jar Sets may become worn depending on the frequency o...

Page 39: ...To protect the operator the drive motor of the TissueLyser II will only start if the transparent hood has been lowered If the START key is pressed while the hood is raised F04 is displayed and the dri...

Page 40: ...es up to 31 C linearly decreasing down to a relative humidity of 50 at 40 C Place of operation For indoor use only Altitude Up to 2000 m 6500 ft Pollution level 2 Transportation conditions Air tempera...

Page 41: ...eled bin symbol see below indicates that this product must not be disposed of with other waste it must be taken to an approved treatment facility or to a designated collection point for recycling acco...

Page 42: ...ment contact your local QIAGEN sales office for the required return form Once the form is submitted you will be contacted by QIAGEN either to request follow up information for scheduling collection of...

Page 43: ...ser Manual 05 2016 43 Declaration of Conformity Name and address of the company QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up to date Declaration of Conformity can be requested from QIAGEN T...

Page 44: ...the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfa...

Page 45: ...TissueLyser II User Manual 05 2016 45 Notes...

Page 46: ...TissueLyser II User Manual 05 2016 46 Notes...

Page 47: ...016 47 Trademarks QIAGEN QIAGEN Group DNA ExitusPlus AppliChem Gigasept Lysetol Mikrozid Sch lke Mayr GmbH DECON QUAT Veltek Associates Inc Teflon E I du Pont de Nemours and Company HB 0262 004 2009 2...

Page 48: ...TissueLyser II User Manual 05 2016 48 1102664 Ordering www qiagen com contact Technical Support support qiagen com Website www qiagen com...