RainDrop Sense Operator’s Manual
LCN 50-04344 Rev. D
5-2
Introduction
Perform the operations described in this chapter on the RainDrop
®
Sense instrument. This
procedure requires a number of consumables (available from RainDance Technologies
®
and
other manufacturers).
Note:
For the list of required consumables, see
“RainDrop® Consumables and Equipment”
This chapter describes how to start a run on the RainDrop Sense instrument. Some of the
steps involve preparing the physical components of the system and some of them take place
within the RainDrop ICS.
For detailed information the ICS software, see
and
.
Note:
Extraneous clusters have been noted in 50ul samples run in the FAST mode on the
Sense instrument. For the highest quality data we recommend limiting sample volumes to
25ul if the samples are to be run in the FAST mode on the Sense instrument.
Gathering Supplies
To start a run on the RainDrop Sense instrument, have the following equipment and supplies
available:
Gloves, nitrile or latex
Sense Chip, P/N 30-06086
Sense Chip Compression Plate, P/N 30-06423
Drive Oil, shipped in syringes, P/N 30-07117
PCR tube strip containing emulsion generated on the RainDance Source instrument
and following thermal cycling.
For Standard Mode detection, standard PCR Tube Strip Caps (P/N 40-06087)
For Fast Mode detection, High Speed Caps (P/N 40-08286)