C2
Epi-illumination swivel filter holder:
The swivel filter holder is screwed into the side of the microscope stand. It contains the
following filters which can be combined with the filters of the epifluorescence
illuminators:
Markings on the swivel arms:
S (white).…..... Empty special filter (2-11mm thick)
I (black)...……. Shield, to block the exciter radiation
I (white)…….... Red blocking filter 50x4 BG 38/h
FITC 490 or UG 1 if image too bright
II (white).......... Yellow filter 50x1 GG 475
To FITC 490 for narrow band exciter
III (white)…...... Empty special filter (2-4mm thick)
For micro photography, an electrical lock can be fitted into the swivel filter holder.
Field iris diaphragm, illumination aperture for microphotometry:
There is an iris diaphragm in the epifluorescence illuminator light pipe. This is adjusted
to prevent UV fading of large areas of the specimen. Opening the field iris diaphragm is
done by rotating the knurled ring, while focusing is done by loosening the hex locking
pin and using it to slide the cylindrical part towards or away from the observer, then
retightening the pin.
In the light pipe is an opening for inserting an aperture slide with a hole of desired size
for microphotometry. These apertures are used when measuring the smallest object
details, and are centered and focusable.
Filter Number
Exciter Filter
Interference Mirror
Blocking Filter
Use
40 (white)
UV exciter filter 18x2 UG 1
(Broadband)
UV reflecting up to 400 nm
18x3 KV 418/VG
For stimulation of tissues in
which it wants to locate
FITC provided that
autofluorescence is desired.
Generally weak
fluorescence.
49° (blue)
Blue exciter filter 18x(3.5
FITC 490 +
2 GG 400) broadband
Blue reflective until approx.
490 nm
18x3 (λ= 525nm)/VG
Double staining for the
presentation of FITC alone
49 (blue)
Blue exciter filter 18x(3.5
FITC 490 + 2 GG 400)
broadband
Blue reflective until approx.
490 nm
18x3 (GG 9 + OG 515)/VG
For FITC alone or for double
staining, if FITC and TRITC
will be visible at the same
time.
56 (green)
Green exciter filter 18x4
(λ=546 nm)/VG
Narrow band
Green reflective until
approximately 660 nm
18x3 OG 590/VG
Rhodamine and derivatives
Light filters in the epifluorescence illuminator for immunofluorescence:
Exciter filter, interference beamsplitter mirror and blocking filter are coupled to each other and can be adjusted at the
same time with the help of the lever located on the front panel. The filter numbers derive from the long wavelength
end of the range of reflection of the interference beamsplitter mirror in nm divided by 10 (nm/10)
Summary of Contents for Univar
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