5
TROUBLESHOOTING
Optical
Problem
Possible Cause
Dust or dirt in field of view.
Poor image (low contrast or resolution)
Uneven Focus
Focusing is not possible with high magnification objectives
Insufficient pafocality of objectives
No cohesion of binocular image
Dust or dirt on objective, filter, condenser or eyepiece
Condenser is set too low or too high
Aperture diaphragm closed too far
No cover glass
Too thick or thin cover glass
Immersion oil not used on immersion procedure
Air bubbles in immersion oil
Immersion oil used on a dry objective
Greasy residue on eye lens
Incorrect illumination
Specimen holder not fixed securely on stage
Specimen not secured in position
Specimen tilted on stage
Slide is upside down
Cover glass is too thick
Eyepiece diopter not adjusted
Eyepiece diopter not adjusted
Magnification or field of view of left and righ eyepiece
differ
Interpupillary distance not adjusted
Eyepiece diopter not adjusted