OPERATION
Carl Zeiss
Lighting and contrasting method in reflected light
Axio Lab.A1
102 430037-7144-001
04/2013
x
For specimens with medium contrast characteristics, set the aperture diaphragm with the knurled
wheel (Fig. 4-19/
1
) to about 2/3 to 4/5 of the exit pupil diameter of the objective.
In most applications, this aperture diaphragm setting provides optimum contrast at almost ideal
resolution, and is therefore the best compromise for the human eye.
x
Then reinsert the eyepiece, adjust the focus with the coaxial coarse and fine focusing knob and adjust
the brightness to the reflected light specimen.
Never use the aperture diaphragm for controlling image brightness. Use the adjusting knob
(Fig. 4-19/
4
) for illumination intensity!
1
Knurled wheel of aperture diaphragm A
2
Knurled wheel of luminous-field diaphragm F
3
Nosepiece
4
Light intensity control
5
Focusing
drive
6
Reflector
turret
Fig. 4-19
Microscope settings in reflected light brightfield
