OPERATION
Axio Lab.A1
Lighting and contrasting method in transmitted light
Carl Zeiss
04/2013 430037-7144-001
77
(3) Setting transmitted light phase contrast
x
Swivel the phase contrast objective, e.g. labeled
Ph 1
, into the beam path.
x
On the turret disk of the condenser, swivel in the phase stop to match the phase contrast objective,
e.g. 1.
x
To check the centering and the congruence of the bright phase stop (in the condenser) with the dark
phase ring (in the objective), remove one eyepiece from the tube and replace it with the centering
telescope. Using the centering telescope's correction facility, focus on the phase stop and the phase
ring in the exit pupil of the objective.
x
If congruence is not perfect (Fig. 4-4/
A
), use the
two SW 1.5 hexagonal screwdrivers (Fig. 4-3/
1
and
4
) to turn the two centering screws
(Fig. 4-3/
2
and
3
) to recenter the bright phase
stop until complete congruence with the dark
phase ring is achieved (Fig. 4-4/
B
).
x
Finally, remove the auxiliary microscope from
the tube and reinsert the eyepiece.
To enhance the image contrast, an interference
wide-band filter, green 32 x 4, can be inserted on
the luminous-field diaphragm or in the color glass
holder (if existing).
Perfect phase contrast is only achieved if the bright
phase stop (in the condenser) and the dark phase
plate (in the objective) are precisely congruent in
the illumination beam path (Fig. 4-4/
B
).
Fig. 4-4
Centering the phase stop (bright
ring in condenser) to the phase ring
(dark ring in objective)
