OPERATION
Carl Zeiss
Lighting and contrasting method in transmitted light
Axio Lab.A1
92 430037-7144-001
04/2013
Evaluation:
If the crystal needles parallel to the gamma direction are yellow and those perpendicular to the gamma
direction are blue, they are monosodium urate crystals (gout).
If the crystal needles parallel to the gamma direction are blue and those perpendicular to the gamma
direction are yellow, they are calcium pyrophosphate crystals (pseudogout).
4.1.6.3
Determining the direction of oscillation n
JJ
(1) Application
The determination of the direction of oscillation of n
J
and n
J
'
(direction of oscillation with the absolutely
or relatively highest refractive index) and n
D
and n
D
'
(direction of oscillation with the absolutely or
relatively lowest refractive index) in relation to the morphological directions, e.g. of crystal surfaces,
crystal needles or fibers, provides an important criterion for recognition. It is also employed for the
diagnosis of biocrystals (e.g. gout, pseudogout).
Fig. 4-14
Determining the direction of oscillation n
J
’
using a synthetic fiber as an example
(2) Equipment configuration for Axio Lab for conoscopy
Eyepiece with crossline reticle
Tension-free objectives
Rotary stage Pol (Fig. 4-12/
1
)
Polarizer D (rotatable or fixed)
Compensator lambda or lambda/4 as required
Alignment specimen for polarization microscope (453679-0000-000)
