6
4.2. General care and maintenance
• Before use clean and dry the apparatus with
distilled water only
.
Important: Acrylic plastic is not resistant to aromatic or halogenated
hydrocarbons, ketones, esters, alcohols (over 30 %) and acids (over 25 %).
• Before use, and then on a monthly basis, check the unit for any leaks at the
bonded joints. Place the unit on a sheet of dry tissue and then fill with
distilled
water only
to the maximum fill line. Any leakage will cause dampening of the
tissue paper. If any leakage is seen
do not attempt to repair or use the
apparatus
, but notify
SERVA Electrophoresis GmbH
Heidelberg resp. the
SERVA Electrophoresis GmbH
distributor immediately.
• The platinum electrodes are partially shrouded for protection. However, when
cleaning the main tank
do not
use cleaning brushes in the electrode area. Usually
a thorough rinse with distilled water is all that is required.
• Ensure that the connectors are clean and dry before usage or storage.
4.3. Buffer volume/running conditions
The buffer volume can be seen in Table1. The running conditions (Table 2) vary
according to the number of gels and their composition. The current required will increase
in proportion to the number of gels providing that the voltage is not limiting, e. g. two gels
require twice the current of one but the same voltage.
By increasing the gel concentration the electrical resistance is increased and the rate of
migration decreases. Higher voltages can be applied but be careful not to overheat the
gel.
Table 2: Running conditions using BlueVertical
TM
in different electrophoresis applications
Type of electrophoresis
Type of SERVA
Gel
TM
Precast Gel
Running conditions
SDS PAGE
SERVA
Gel
TM
HSE
•
Volt
const.
: 400 Volt
•
Time: 20 minutes
SDS PAGE
SERVA
Gel
TM
TG PRiME
TM
•
Volt
const.
: 300 Volt
•
Time: 35 minutes
SDS PAGE
SERVA
Gel
TM
TG Neutral
•
Volt
const.
: 150 Volt
•
Time: 70 minutes
Native PAGE
SERVA
Gel
TM
N
•
Volt
const.
: 130 Volt
•
Time: 60 – 120 minutes
Isoelectric Focusing
SERVA
Gel
TM
IEF
•
Volt
const.
: 100 Volt for 60 minutes
•
Volt
const.
: 200 Volt for 60 minutes
•
Volt
const.
: 500 Volt for 30 minutes
•
Time: 150 minutes
For detailed information about the running conditions of SERVA
Gel
™precast vertical mini
gels, please see the manuals supplied with the gels.
DOMINIQUE DUTSCHER SAS
