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ver.220216E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 Asahipak

 ES-502C 7C 

 

(Please read this manual carefully to achieve the best and consistent column performance for a long time) 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 Asahipak

 ES-502C 7C is a week cation 

exchange column filled with hydrophilic polymer-base gel modified with carboxymethyl group. It is an ideal 
column for analyzing various proteins, peptides, and components of nucleic acids, because of its small 
hydrophobic adsorption. The polymer-based gel allows its use at a wide pH range condition. 

   

 

2. Column Components   

 
 

               

 

 

 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle 

Size  

(

μ

m) 

Theoretical Plate 

Number 

(Per Column) 

Ion Exchange 

Capacity       

(meq/g) 

I.D. Length 

F7640001 

Asahipak ES-502C 7C 

7.5 100 

 3,300 

0.55 

 

Base Material 

: Spherical porous particles of polyvinyl alcohol modified with carboxymethyl group

 

Column Housing  : SUS-316   
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : 0.1 M Sodium Phosphate Buffer (pH4.4) 

 
 
 
 
 

4.

 

Usable Conditions 

Summary of Contents for Asahipak ES-502C 7C

Page 1: ...l number written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex Asahipak ES 502C 7C is a week cation exchange column filled with hydrophilic polymer base gel modified with carboxymethyl group It is an ideal column for analyzing various proteins peptides and components of nucle...

Page 2: ...rosive salts such as sodium chloride wash out the salts at the end of analysis The metal parts of the devices and or the columns may rust Column pressure is influenced by the eluent composition flow rate and column temperature When changing the eluent compositions adjust the flow rate and column temperature so that the column pressure remains below the usable maximum pressure 5 Eluent Preparation ...

Page 3: ...the column to LC system by following the flow direction arrow indicated on the column name tag 2 Make sure to insert the tubing all the way to the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the column side of the end fitting Presence of an extra space will let the sample to spread out and may result in wide peaks 3 Set the initia...

Page 4: ...ethod 1 Insoluble components that block the column inlet may be removed by reversing the flow direction i e introducing the eluent from the column outlet with flow rate at less than half of the recommended flow rate 2 Follow below cleaning steps for adsorbing components For an efficient cleaning reverse the flow direction and use the flow rate at less than 0 5 mL min Introduce 5 to 10 times column...

Page 5: ...r N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support office https...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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