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ver.211228E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 Asahipak

 GF-HQ series 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex™ product. Shodex

 Asahipak

 GF-HQ series is polymer-based size 

exclusion chromatography columns. They work well with both aqueous and organic solvents.   
 

2. Column Components

                 

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
3. Column Specifications 

 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle 

Size (

μ

m) 

Theoretical 

Plate Number 

(Per Column) 

Target Molecular 

Weight Range

*1

 

Exclusion 

Limit

*1

 

I.D. Length 

 

F7600000

 

Asahipak GF-210 HQ 

7.5

 

300

 

5

 

 19,000 

300 - 4,000 

      9,000 

F7600001

 

Asahipak GF-310 HQ

 7.5

 

300

 

5

 

 19,000   

300 - 30,000 

    40,000 

F7600002

 

Asahipak GF-510 HQ

 7.5

 

300

 

5

 

 19,000 

5,000 - 200,000 

  300,000 

F7600004

 

Asahipak GF-7M HQ 

7.5

 

300

 

9

 

 13,000 

300 - (10,000,000)

*2

 (10,000,000)

*2

 

F6710018

 

Asahipak GF-1G 7B 

7.5

 

 50

 

9

 

(Guard Column) 

 

GF-7M HQ is a mixed-gel column, suitable for the analysis of wide molecular weight ranges. 

 

 *1

 Reference value only / Measured with pullulan 

*2

 Estimated value 

 

 
 
 
 
 

Summary of Contents for Asahipak GF-HQ Series

Page 1: ...ial number written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex Asahipak GF HQ series is polymer based size exclusion chromatography columns They work well with both aqueous and organic solvents 2 Column Components 3 Column Specifications Product Code Product Name Column Siz...

Page 2: ...ium sulfate Please keep total concentration of salts under 0 5 M Their recommended concentration ranges are 0 05 0 3 M 2 Salts such as lithium bromide can be added to DMF and DMSO Their recommended concentration ranges are 10 50 mM 3 Aqueous solutions of protein denaturants such as urea and guanidine hydrochloride can also be used However since highly concentrated solutions are usually used there ...

Page 3: ...ould not be used The content may have been changed absorbed moisture or has been contaminated Always use freshly prepared solvents Solvents stored for a long time may have changed their compositions and may influence elution patterns and or damage the column Note Use of on line degasser is recommended 6 Sample Preparation 1 If possible use the eluent for analysis to dissolve or dilute samples If t...

Page 4: ... If using the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 Multiple number of columns can be connected in series When connecting multiple columns with each having different exclusion limits set the column with higher exclusion limit at the upper stream position Wh...

Page 5: ...cal column alone If the problem is solved most likely the cause is from the guard column In this case clean the guard column If the problem is not solved by removing the guard column clean both guard and analytical columns Make sure to clean the guard and the analytical columns separately When washing the column let the washing solution flow from the column outlet go directly into the waste contai...

Page 6: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Page 7: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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