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Operation Manual

Shodex Asahipak NH2P-50 10E, Operation Manual

The Shodex Asahipak NH2P-50 10E is a high-quality chromatography column known for its exceptional performance and versatility. To ensure optimal usage, don't forget to consult the comprehensive Operation Manual available for free download at 88.208.23.73:8080. This detailed manual highlights key instructions and guidelines, facilitating seamless operation of this remarkable product.

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Operation Manual Shodex Asahipak NH2P-50 10E, NH2P-90 20F, NH2P-130G 7B

Version 150305E

Operation Manual

Shodex™

Asahipak

™

NH2P-50 10E,

NH2P-90 20F, NH2P-130G 7B

Europe, Middle East, Africa, Russia
www.shodex.de

Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany

Technical Support:

info@shodex.de
+49 (0)89 / 93 99 62 37

Quotes and Orders:

order@shodex.de
+49 (0)89 / 93 99 62 34

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Summary of Contents for Asahipak NH2P-50 10E

Page 1: ...l Shodex Asahipak NH2P 50 10E NH2P 90 20F NH2P 130G 7B Europe Middle East Africa Russia www shodex de Sales Office Showa Denko Europe GmbH Shodex Business Konrad Zuse Platz 3 81829 Munich Germany Technical Support info shodex de 49 0 89 93 99 62 37 Quotes and Orders order shodex de 49 0 89 93 99 62 34 ...

Page 2: ...ure due to leakage from the column or adjoining tubing Attention Use the column within the regular range of flow rate pressure and temperature The column performance may deteriorate when it is handled beyond the permissible range even for a short time See the Section 4 Usable Conditions for the permissible ranges 3 Specifications Product code Product name Size mm Particle size μm Plate Number per ...

Page 3: ... the column tag Set the flow rate at half of standard rate and connect the column Flow at the low rate until the column temperature increases to the setting temperature and then increase the flow rate to the analytical condition Attention The column should always be installed in the manner above for safe and effective operation 7 Eluents HILIC mode IEC mode Operational solvents Aqueous solutions o...

Page 4: ...peration below This equilibrium operation is not needed when acetonitrile water or buffer eluents are used HILIC mode IEC mode Eluent Acetonitrile buffer Acetonitrile water Buffer Solution The same buffer without acetonitrile Unnecessary Unnecessary Quantity 10 to 20 times the column volume Flow rate Lower than half of the normal flow rate 8 Sample Preparation HILIC mode IEC mode Sample Preparatio...

Page 5: ... Eluent 95 min 3 min 138 min 1 min Caution Do not mix the HClO4 solution with the NaOH solution as NaClO4 is produced when mixed NaClO4 is a combustible and explosive compound Flow the acidic and alkali waste into separate bottles never concentrate or dry the wastes and process each waste promptly Attention Complete the washing procedure steps continuously Do not store the column with either acid ...

Page 6: ...ade in any of the following cases 3 1 If the Shodex Column is not used in accordance with the operating manual 3 2 If the Shodex Column is remodelled by anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex Column is disposed of 3 4 If the Shodex Column is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex Colum...

Page 7: ...ak VT Quaternary Ammonium NR3 For polar anionic compounds like glyphosate phosphorylated sugars HILIC anion exchange mode HILICpak VC Carboxyl COO For polar cationic compounds like choline acetylcholine amino acids neurotransmitters Pharmaceuticals like metformin HILIC cation exchange mode HILICpak VN Diol OH For oligosaccharides up to DP25 like hydrolysed cellulose or dextran For biomolecules oli...

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