ver.210629E
SHOWA DENKO K.K. (https://www.shodex.com/)
2
4.
Usable Conditions
*
The modified sulfo group may act as a decomposition catalyst for some sugars including sucrose and raffinose. The effects
is larger at the higher temperature (more decomposition). In case analysis of those sugars cannot be avoided, it is
recommend to set the column temperature at 40 °C or below.
Usable solvents are listed below.
(1) The standard eluent is acidic aqueous solution.
(2) Usable acids include sulfuric acid and phosphoric acid. For sulfuric acid, the standard concentration is 5 mM
and the maximum concentration is 25 mM.
(3) Organic solvents cannot be added.
Caution!
· After using a strong acidic eluent such as sulfuric acid solution, wash the HPLC system
and the column thoroughly. If left, the metal parts of the system and/or the column may
react with the acid and generate hydrogen gas.
Attention!
· Use the column within above stated flow rate, pressure, and temperature ranges. Using
the column outside the given range may damage the column and lower its performance.
·
Column pressure is influenced by the eluent composition, flow rate, and column
temperature. When changing the eluent compositions, adjust the flow rate and column
temperature so that the column pressure remains below the usable maximum pressure.
· Do not use hydrochloric acid as it corrodes stainless steel.
5. Eluent Preparation
(1) Degas the eluent fully to prevent the formation of air bubbles.
(2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear
as noise on the chromatograms. Filter the eluent with a 0.45-
μ
m disposable filter to prevent the problems.
Attention!
· Whenever water is required, use ultra-pure water freshly generated by a water purification
system or water from a newly opened HPLC grade distilled water bottle. Solvents left in an
opened bottle for a long time should not be used. The content may have been changed or
has been contaminated.
· Always use freshly prepared solvents. Solvents stored for a long time may have changed
their compositions and may influence elution patterns and/or damage the column.
Note
· Use of on-line degasser is recommended.
6. Sample Preparation
(1) If possible, use the eluent for analysis to dissolve or dilute samples. If this is difficult, use a solvent which
has a composition that is as close as possible to the eluent's composition, but which fully dissolves or dilutes
the sample. When gradient elution is used, it is recommended to use the initial eluent to prepare the sample.
(2) Filter the sample solution using disposable 0.45-
μ
m filter to prevent the column from clogging or deteriorating.
(3) Recommended sample injection volume is less than 10
μ
L per column.
(4) When analyzing a basic sample, make sure to neutralize the sample prior to the injection.
(5) When a sample contains cations (the pretreated sample after neutralization), remove them by using a cation
exchange resin.
(6) When a sample contains protein or lipid, make sure to remove them prior to the injection. Proteins may be
removed by ultrafiltration or by adding acid or acetonitrile.
(7) When a sample contains hydrophobic substances or surfactants, use a reversed-phase solid phase
extraction to remove them.
Product Name
Flow Rate (mL/min)
Maximum Pressure
(MPa) (Per Column)
Temperature (°C)
Recommended Maximum
Recommended
*
Maximum
SUGAR SH1011 8C
0.5 - 1.0
1.5
1.5
50 - 60
95
