Singer Instruments ROTOR HDA, User Manual

Page 1: ...User Guide v1 2...

Page 2: ...R HDA require moving During Pad Head removal always ensure the push cylinder is cleared before moving away from the carriage The push cylinder runs from the carriage arm into The Stinger Pad Head If e...

Page 3: ...tra fast manipulation of high density arrays of yeast other fungi and bacteria Reagent sets such as deletion mutant collections and the complete set of cloned yeast genes can be utilised for high thro...

Page 4: ...en Protective Screen Screen Handle Lid Lifters Turntable Hopper Control Panel Pad Head Emergency Stop Carriage Arm RS232 Shuttle Input Dump Zone Hopper Loading Bay Black Bay Dump Drawer Fast Buttons U...

Page 5: ...my features Beer Bottle Opener Air Line Connection NOTE Ensure to keep transit hardware in a safe place should the ROTOR HDA require moving Removable Transit Clamps Removable Transit Bar MCI Mounting...

Page 6: ...er is where the fresh RePads are loaded Load a stack of RePads pins facing down into the Hopper as shown ensuring the chamfered corner is to the top right The Pad Hopper is fully autoclavable to ensur...

Page 7: ...Solid agar Solid agar Solid agar Solid agar Liquid Solid agar 96 short Compatible densities Compatible densities Compatible densities Compatible densities Compatible densities Compatible densities Co...

Page 8: ...irst The turntable will then rotate to allow you to load the second two The Pad Head moves to the Source Plate lowers and collects a sample of cells The Pad Head lowers and picks up a RePad The Pad Ho...

Page 9: ...repeated until your chosen protocol is finished Follow the simple step by step instructions for loading unloading of plates and consumables See page 14 The Pad Head moves to the Dump Zone and drops t...

Page 10: ...o work through the ROTOR HDA initialisation procedure Turn on the Compressor Select OK on the Touch Screen or use the Fast Buttons Open the Roll over Cover OK Cancel Insert the Power Cables into the R...

Page 11: ...ents com 4 5 Initialisation routine Clear all objects from the ROTOR Stage so nothing will obstruct the initialisation routine Select OK On screen or use the Fast Buttons The ROTOR HDA will perform a...

Page 12: ...s on The source could be colonies grown on solid agar or cultures grown in liquid media In solid agar the ROTOR HDA currently supports colony density up to 24576 In liquid media the ROTOR HDA supports...

Page 13: ...media The final step is to select the program that you want to perform The programs available are only those compatible with your previous selection In this example there are three available programs...

Page 14: ...the Fast Buttons to continue 3 Simple step by step instructions will guide you through the program Each plate position is colour coded Red blue yellow and green go into the ROTOR Turntable while black...

Page 15: ...he ROTOR HDA will now count the number of Pin Pads loaded to ensure there are enough to complete the program The ROTOR HDA will now start pinning the colonies for replication Live program information...

Page 16: ...than 4 replicates of a target plate you can repeat the program with one click Make your selection When pinning is completed you will be instructed to remove the Plates Press OK when you are done A Pro...

Page 17: ...Hopper from the Home Screen Rotate This rotates the Turntable Reset This resets the ROTOR HDA Load Plate Remove Plate Swap Plates UV This opens the UV Lamp Options Info This opens the Info and Settin...

Page 18: ...y removed when it is vital to do so This will increase the time it takes to run each program but each source plate will be exposed for less time and the print head will never move over a source plate...

Page 19: ...lates The retraction distance applied to the Pad Head after sensing the bottom of the plate The number of times each source pin ning will repeat Skipping around on the agar surface to select from a wi...

Page 20: ...for each source pinning This feature is useful when pinning from source plates that have been printed to a non central location The pressure that the Pad Head will use to push onto the agar The speed...

Page 21: ...he diameter from the specified diameter uniformly with each cycle for a thorough mix The number of cycles comprising of 5 steps that the dry mix on source plates will be executed Liquid mixing can be...

Page 22: ...linear encoder Y Z are open loop stepper motor drives with optical data setting Pad Head Vacuum operated and fully floating to comply with agar surface Programmable variable pressure Pad Dispenser Ho...

Page 23: ...lst replication is in progress This makes the process very continuous Performance tests carried out for Singer by a major yeast laboratory claim replication rates in excess of 100 PlusPlates per hour...

Page 24: ...ed onto 1x 384 density plate 1 2 Pinning examples 1 4 Array 1 4 Single source 1 4 single source Each colony from a 1x 96 density plate replicated in quadruplicate to a 1x 384 density plate These proto...

Page 25: ...HDA Success Time to enjoy your Delicious Beer you ve earned it Repeat steps 1 6 until suitably relaxed Place the Bottle Cap in the Bin Nobody likes a litter bug Lever the Beer Bottle to remove the Bot...

Page 26: ...26 Notes...

Page 27: ...27 singerinstruments com Notes...

Page 28: ...28 Notes...

Page 29: ...29 singerinstruments com Notes...

Page 30: ...Roadwater Watchet Somerset TA23 0RE UK 44 0 1984 640226 tel 44 0 1984 641166 fax contact singerinstruments com singerinstruments com Scan to visit website for more helpful tips and tutorials...