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8. Test Instructions
8.1 Biological performance of sterilizers
It is commonly used as a challenge organism for sterilization validation studies and periodic
check of sterilization cycles. The biological indicator contains spores of the organism on filter
paper inside a vial. After sterilizing, the cap is closed, an ampoule of growth medium inside of the
vial is crushed and the whole vial is incubated. A color and/or turbidity change indicates the
results of the sterilization process; no change indicates that the sterilization conditions were
achieved; otherwise the growth of the spores indicates that the sterilization process has not been
met.
An example of Raven ProTest is description as following:
1. Please put one or more Raven ProTest units in a horizontal position in the most difficult to
sterilize locations and run cycle.
Warning:
After sterilization, handle unit (biological indicator) with care.
Warning:
Raven ProTest is trademark of Mesa Laboratories, Inc.
1. After the Biological indicator has cooled, crush the media ampoule by squeezing the sides of
the plastic tube or by using the tool provide.
2. Place processed unit(s) and one unprocessed (control) unit in a vertical position in an
incubator at 58-62
℃
for steam (Geobacillus steaothermophilus) for 24hours.
3. Begin monitoring the incubated units after 12-18hours. Record observations.
4. The control unit should exhibit turbidity and/or color change to or toward yellow.
5. A fail sterilization cycle is indicated by turbidity and/or color change to or toward yellow. A test
unit that retains its original color indicates the sterilization parameters have been met.
6. More detail information please asks your dealer of biological test.
