Sutter Instrument Lambda DG-4Plus/USB, Operation Manual

Page 1: ...949 Voice 415 883 0128 Web www sutter com Fax 415 883 0572 Email info sutter com Lambda DG 4Plus USB and Lambda DG 5Plus USB Ultra High Speed Wavelength Switching Xenon Arc Light Source System Operati...

Page 2: ...BDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 ii Copyright 2019 Sutter Instrument Company All Rights Reserved LAMBDA 10 and SmartShutter are registered trademarks of Sutter Instrument Compa...

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Page 5: ...US USB and DG 5PLUS USB using 100 240 V AC 50 60 Hz line voltage This instrument is designed for connection to a standard laboratory power outlet Overvoltage Category II and because it is a microproce...

Page 6: ...window of the CERMAX lamp Note that the lamp output is towards the heat sink mounted on the side of the cabinet Avoiding Physical Injury while Powered Up and Emitting Light WARNING DO NOT LOOK DIRECTL...

Page 7: ...for further information There are no ventilation requirements for the ozone free bulb Avoiding Physical Injury and Equipment Damage while Replacing the Xenon Bulb WARNING Before removing the xenon arc...

Page 8: ...ncinerator SAFETY HAZARDS The operation of lamps involves one or more of the following hazards In the absence of safe operating practices and precautions any one of these hazards could result in injur...

Page 9: ...5PLUS and the DG 4 5PLUS USB 2 1 4 Lambda DG 4PLUS USB and Lambda DG 5PLUS USB 3 1 4 1 Systems 3 2 INSTALLATION 5 2 1 Unpacking 5 2 2 Getting Started 5 2 3 Light Guide Optical Holder Installation 8 2...

Page 10: ...8 4 Running the RING BUFFER Sequence 31 3 7 9 Turbo Blanking Mode 32 3 7 10 Display the First Four Filter Values Mode 33 4 EXTERNAL CONTROL OPERATIONS 35 4 1 Parallel Interface 46 4 1 1 Connecting to...

Page 11: ...1 Light guide installation 6 Figure 2 2 Lambda DG 4 left side 7 Figure 2 3 Liquid light guide installation 9 Figure 2 4 Light guide optical holder 9 Figure 2 5 Filter holder assembly 10 Figure 2 6 Fil...

Page 12: ...Commands no return data 38 Table 4 5 Special commands reference 39 Table 4 6 Discontinued commands USB equipped DG 4 DG 5 Plus 45 Table 4 7 Parallel port pin assignments 46 Table 4 8 Command code type...

Page 13: ...of user interaction with the system Manual local control and external computer control Please take the time to read these instructions to assure the safe and proper use of this instrument 1 1 2 Techni...

Page 14: ...r optical paths An additional slide in filter holder intended for neutral density filters is located in a portion of the optical system common to all light paths After passing through the filter posit...

Page 15: ...ly to the Lambda DG 5PLUS The only difference between the Lambda DG 4PLUS USB and Lambda DG 5PLUS USB is the set of light interference filter holders as follows The Lambda DG 4PLUS USB filter set cons...

Page 16: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 4...

Page 17: ...ing spacer ring and blanking disc Power Cord Liquid Light Guide USB Interface Cable 9 pin Serial Interface Cable 25 pin Parallel Interface Cable 25mm Cylindrical Spanner Wrench 18mm Flat Spanner Wrenc...

Page 18: ...abinet to ventilate the ozone from the xenon lamp to the environment or to an ozone removal device 5 Identify the small access door used to install the neutral density FILTER HOLDER This access door i...

Page 19: ...eyes DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE 8 Turn on the power to the lamp subsystem using the switch labeled LAMP on the back panel of the Lambda DG 4 cabinet Due to the current draw during lamp...

Page 20: ...utter and the four FILTER POSITIONS with minimal attenuation respectively Now that you have completed the Make it Glow section of the manual you should be familiar with the basic components and operat...

Page 21: ...uid light guide installation 2 3 3 Optical Holder The free end of the light guide slides into one end of the optical holder tube shown below The other end of the optical holder tube has a UV grade fus...

Page 22: ...s as well as the filters Figure 2 5 Filter holder assembly The threaded retaining ring has two notches cut into it 180 degrees apart Place the notched end of the brass spanner wrench into the notches...

Page 23: ...nly be removed from those positions that will contain a filter The discs are intended as a safety device for the occasion where you may be looking into the microscope We strongly urge that the discs b...

Page 24: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 12 Figure 2 7 Lambda DG 4 left side...

Page 25: ...icroscope The intensity and wavelengths of the light that can be produced by the Lambda DG 4 are such that it should not be turned on unless the light is directed into a microscope and away from the e...

Page 26: ...on If the unit is under remote control at power on the appropriate remote interface mode will be automatically set and the display may change immediately to reflect any waiting computer commands PARA...

Page 27: ...DE after the ON LINE key has been pressed unless the SERIAL PORT is active ENTER This key is used in conjunction with the numeric keys for selecting FILTER NUMBERS and entering other numeric data It i...

Page 28: ...ed simultaneously with the serial port cable 3 5 2 Serial Port This 9 pin DSUB connector is used for establishing a serial interface between the Lambda DG 4 and a remote device computer using the cabl...

Page 29: ...This socket is used to connect the power cord provided with the Lambda DG 4 3 6 Fuse The fuse used in this instrument depends on the power rating of the installed lamp and mains voltage See APPENDIX C...

Page 30: ...he operational modes will be described in terms of LOCAL control Separate sections discussing interfacing the Lambda DG 4 with remote devices computers will cover access to these same modes of operati...

Page 31: ...y programmed for that FILTER NUMBER value see the section on SETUP modes Under LOCAL control the filter is selected by entering a value in the range of 0 to 15 If a single digit value is desired the u...

Page 32: ...DG 4 In this way filter changes can be coordinated to occur with the capture of video frames The FILTER NUMBER sequence can be programmed at your convenience and then executed later with precise timin...

Page 33: ...can be programmed at your convenience and then executed later with precise timing see SETUP THE RING BUFFER The FILTER NUMBERS can also be sent individually via the serial or parallel port before the...

Page 34: ...ny with the next vertical sync pulse that occurs The computer interfaced with the Lambda DG 4 must be set up to monitor the video signal and hold the STROBE line HIGH if the number of frames to be cap...

Page 35: ...d The Input Galvanometer directs the collimated light beam through one of the four FILTER POSITIONS shown below and thus through whatever interference filter is installed there The angle of a Galvanom...

Page 36: ...POSITION Table 3 1 Input and output galvanometer values based on filter position Filter Position Input Galvanometer Output Galvanometer 1 3500 750 2 2500 1500 3 1500 2500 4 750 3500 3 7 7 2 Filter Num...

Page 37: ...ter position The actual attenuation produced must be determined by the user The degree of attenuation produced by increasing the galvanometer value is similar to that produced by an equivalent decreas...

Page 38: ...FINITION menu Alternatively you can page through the mode selection menus by pressing MODE The following menu will be displayed Press 1 three times to scroll through the NEXT TWO PAGES to see the desi...

Page 39: ...pplication This could be accomplished by repeatedly reprogramming the FILTER NUMBER you wish to evaluate but that would prove quite tedious Direct control of the Output Galvanometer Value assigned to...

Page 40: ...and released to increment or decrement the value slowly or they can be held down continuously for more than 2 seconds to rapidly increase or decrease the value When you are finished press the ENTER k...

Page 41: ...ressing MODE The following menus will be displayed Press 1 three times to scroll through the NEXT TWO PAGES to see the desired option or press the 0 key to abort the routine and return to the MAIN MEN...

Page 42: ...ernatively you can page through the mode selection menus by pressing MODE The following menus will be displayed Press 1 three times to scroll through the NEXT TWO PAGES to see the desired option or pr...

Page 43: ...he Lambda DG 4 To manually RUN the RING BUFFER access the SETUP RING BUFFER menu by pressing the MODE key followed by the 8 key Alternatively you can page through the mode selection menus by pressing...

Page 44: ...Port as the filter change occurs This results in a brief output of light at an undesired wavelength prior to arriving at the selected FILTER POSITION This problem can usually be avoided by placing fil...

Page 45: ...e First Four Filter Values Mode MODE 3 To display the current Input and Output Galvanometer Values for the first four FILTER NUMBERS press the MODE key The following menu will be displayed To bring up...

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Page 47: ...ter port 25 pin DSUB connector USB Interface While the serial and parallel interfaces are selectable between them as in earlier models of the DG 4 5 the Plus USB version of the instrument adds the USB...

Page 48: ...byte No delimiters are used between command sequence arguments Every command and command sequence ends with a terminator byte containing an ASCII CR 13 decimal 0D hexadecimal Although most command by...

Page 49: ...7 G 3 Command 50 Move on command to 3rd filter 25mm at half 8 08 00001000 0008 H 4 Command 50 Move on command to 4th filter 25mm at half 9 09 00001001 0009 I 1 Command 33 Move on command to 1st filter...

Page 50: ...d filter 18mm at half 9 09 00001001 0009 I 4 Command 50 Move on command to 4th filter 25mm at half 10 0A 00001010 0010 J 5 Command 50 Move on command to 5th filter 25mm at half 11 0B 00001011 0011 K 1...

Page 51: ...ons of their functions Table 4 5 Special commands reference Command Tx Rx Ver Byte Count Byte Offset Value Alt key pad entry Ctrl char ASCII def char Description Dec Hex Binary Open Shutter Tx All 1 0...

Page 52: ...e accepted as valid commands Rx All 2 0 Echoed command sequence 1 13 0D 0000 1101 CR Completion indicator Activate Turbo Blanking Tx All 1 0 186 BA 1011 1010 0186 Turns Turbo Blanking ON Rx All 2 0 Ec...

Page 53: ...SB 33 0 243 F3 1111 0011 0243 Command byte 1 63 3F 0011 1111 0063 Galvo A or 191 BF 1011 1111 0191 Galvo B 0 4095 0000 FF0F 0000 0000 0000 0000 1111 1111 0000 1111 0000 0000 0255 0015 Filter galvo DAC...

Page 54: ...d command sequence 1 13 0D 0000 1101 CR Completion indicator Run from Ring Buffer Tx All 1 0 241 F1 1111 0001 0241 Begins filter position movement based on what is defined in the ring buffer A trigger...

Page 55: ...0162 Move on rising edge then to Filter 0 on signal falling as default 163 A3 1010 0011 0163 Move on falling edge as default 164 A4 1010 0100 0164 Move on falling edge then to Filter 0 on signal risin...

Page 56: ...fied filter position Rx USB 5 0 Echoed command sequence 2 0 4095 0000 FF0F 0000 0000 0000 0000 1111 1111 0000 1111 Galvo A DAC value encoded into a 16 bit unsigned short 2 bytes Little Endian 4 13 0D...

Page 57: ...format see NOTE 3 The value stored in these two bytes is always unsigned meaning that the value will always be positive negative values are not allowed These two bytes are converted to and from 16 bit...

Page 58: ...Pin Function 1 Not Connected NC 14 NC 2 Command Bit 0 filter 15 NC 3 Command Bit 1 filter 16 NC 4 Command Bit 2 filter 17 NC 5 Command Bit 3 filter 18 NC 6 Command Bit 4 hold for trigger 19 NC 7 Comma...

Page 59: ...00010000 00011111 are also read as filter change commands These commands will not be executed however until the next trigger STROBE or SYNC PULSE depending on the mode that is active has been detected...

Page 60: ...nt to the Lambda DG 4 in the same manner that they are sent to a printer A printer is designed to print at its maximum rate and if the computer generates output at a higher rate the printer will store...

Page 61: ...various adapter cards to be sure of the base address on the adapter you wish to use The connections to the parallel port are made through a DB 25 connector on your computer The pin assignments for th...

Page 62: ...t all If you have determined that your application requires this output to be read from your computer this can be done through the 4 bit input port on the printer adapter The address of this port will...

Page 63: ...nd operation until the value read for the most significant bit is 1 Since the printer adapter inverts this bit this condition will be met only when the BUSY line is low An XOR operation can be used as...

Page 64: ...s Command Tx Rx Ver Byte Count Byte Offset Value Alt key pad entry Ctrl char ASCII def char Description Dec Hex Binary Open Shutter Tx All 1 0 170 AA 1010 1010 0170 Moves the light path away from Filt...

Page 65: ...the serial mode by simply sending the decimal value 238 EE hexadecimal via the serial port Do not send this data value by both the serial and parallel port since this will cause the controller to rapi...

Page 66: ...by echoing the command back to the host computer This indicates that the command was received and will be executed promptly If the sent command is the same as the last command received it will not be...

Page 67: ...t CHR 13 THEN RETURN IF DATANUM 238 THEN RETURN D D 1 IF D 10000 THEN RETURN GOTO SERIN1 SERCLEAR DO WHILE LOC 1 0 SerialInput INPUT 1 1 characters waiting LOOP RETURN Commands DATANUM 170 OPEN SHUTTE...

Page 68: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 56 DATANUM 241 RUN RING BUFFER FIRST SELECT TRIGGER SOURCE DATANUM 242 END RING BUFFER RUN...

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Page 71: ...he bulb assembly has the following components Figure 5 1 Lamp bulb assembly components 1 Blue lamp housing 2 Notch indicating the front light output side 3 Front terminal smaller threads 4 Rear termin...

Page 72: ...cover panel This will expose the blue plastic lamp housing assembly 3 Grasp the top and bottom handles of the blue plastic housing assembly and carefully pull the assembly straight out of the cabinet...

Page 73: ...have one 5 1 3 2 Installing the New Bulb 1 Without removing the protective cap from the new bulb apply a thin even layer of thermal compound to the side surfaces of the anode shell as shown in Figure...

Page 74: ...ulb surfaces requiring they be covered with thermal compound Figure 5 5 Heat sink alignment and positioning of the retaining clips 4 Insert the heat sink bulb assembly into the blue housing Make sure...

Page 75: ...om handles of the blue plastic housing assembly and carefully reinsert the assembly into the cabinet Figure 5 7 with the bulb window facing away from the lamp fan Figure 5 7 Returning the light bulb a...

Page 76: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 64 This page intentionally blank...

Page 77: ...ranty work will be performed only at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after which the cost is the re...

Page 78: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 66 This page intentionally blank...

Page 79: ...in the following do not include the outer blue housing If an extra housing is required please contact Sutter Instrument by phone fax or email O661301 Ozone free 300 Watt xenon bulb attenuated output...

Page 80: ...al Density Filter Accessories DG ND Filter holder X664162 Filter cup B 7 SmartShutter IQ25 DG 25mm SmartShutter with housing to fit Lambda DG 4 DG 5PLUS USB LB SC Dedicated SmartShutter controller sys...

Page 81: ...in a compartment as shown in the figure To remove the spare fuse press down on the end of the compartment and push it out of the other end The old fuse can serve as a convenient tool for pushing the s...

Page 82: ...se requirements according to lamp power ratings and line voltage Xenon Lamp Power Rating Line Voltage 100 240 VAC Fuse Rating Manufacturer Examples RoHS Compliant Lead Free Yes No 300 Watts T6 3A 250V...

Page 83: ...US USB Two 25mm 1 and three 18mm diameter filters Interference Filter Switching Times 0 5 millisecond between adjacent filters to 1 millisecond between the most furthest apart filters Dimensions H x W...

Page 84: ...048 m Fair Rite 0443167 251 See Note 1 Connected to metal faceplates of connectors on both ends See Note 2 Note 1 Fair Rite Products Corp P O Box J One Commercial Row Wallkill NY 12589 USA Note 2 The...

Page 85: ...mm at 1 3 11 0B 00001011 0011 K 3 Command 33 Move on command to 3rd filter 25mm at 1 3 12 0C 00001100 0012 L 4 Command 33 Move on command to 4th filter 25mm at 1 3 13 0D 00001101 0013 M Unused 14 0E 0...

Page 86: ...00001101 0013 M 3 Command 33 Move on command to 3rd filter 18mm at 1 3 14 0E 00001110 0014 N 4 Command 33 Move on command to 4th filter 25mm at 1 3 15 0F 00001111 0015 O 5 Command 33 Move on command...

Page 87: ...nt filter position to Filter 0 where the light is blocked The original filter position is stored so that when the Open Shutter 170 command is issued the light path is moved from Filter 0 back to the o...

Page 88: ...16 bit unsigned short 2 bytes Little Endian Rx USB 5 0 Echoed command sequence 4 13 0D 0000 1101 CR Completion indicator Set Specified Filter s Galvo B DAC Value Tx USB 4 0 243 F3 1111 0011 0243 Comm...

Page 89: ...of filter values to be loaded in the ring buffer A maximum of 100 filter values are allowed all entries in excess of 100 are ignored Rx All 2 0 Echoed command sequence 1 13 0D 0000 1101 CR Completion...

Page 90: ...down default restored at power up 1 1 01 0000 0001 0001 A Move on rising edge 2 02 0000 0010 0002 B Move on rising edge then to Filter 0 on signal falling 3 03 0000 0011 0003 C Move on falling edge 4...

Page 91: ...1 0000 1111 Galvo A DAC value encoded into a 16 bit unsigned short 2 bytes Little Endian 4 13 0D 0000 1101 CR Completion indicator Get Specified Filter s Galvo B DAC Value Tx USB 4 0 244 F4 1111 0100...

Page 92: ...alled or SS NC not installed 16 13 0D 0000 1101 CR Completion indicator NOTES 1 A short delay usually around 1 ms is recommended between commands after the reception of one command and the sending of...

Page 93: ...ord values 4 The following commands used in the original DG 4 DG 5 and DG 4 Plus DG 5 Plus are discontinued in the DG 4 USB DG 5 USB Triggered by Video Sync 204 CC 11001100 Disable Triggered by Video...

Page 94: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 82 NOTES...

Page 95: ...70 external control operations 35 F filter cup 10 cup spacer ring 10 loading 10 manual switching 8 orientation 10 position selection of 11 retaining ring 10 selecting position 11 filter holder access...

Page 96: ...SETUP RING BUFFER 28 TURBO BLANKING 32 operations 13 optical holder installation 9 optical pathway 2 P parallel port connection 16 46 control 48 input lines 46 pin assignment 46 power connection 17 co...

Page 97: ...69 output range 69 type 69 light guide dimensions 69 weight 69 technical support 1 top panel controls 14 trigger connection 17 TURBO BLANKING 11 32 U unpacking 5 USB port connection 16 V vent hose con...

Page 98: ...LAMBDA DG 4 5 PLUS USB OPERATION MANUAL REV 1 01B3 20190607 86 NOTES...