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3. While viewing through the eyepiece, rotate the coarse focusing knob
to bring the specimen into view. Next, sharpen the focus using the
fine focus knob.
4. If the image of the specimen appears pale, the aperture of the
diaphragm should be decreased (if the model has a disc diaphragm,
rotate the disc to a smaller hole, conversely, if the model has the iris
diaphragm, it should be closed slightly). This will increase contrast
in the specimen’s image. If the specimen appears dark, slightly
open the diaphragm.
5. Rotate the nosepiece to the next higher power objective. A slight
turn of the fine focusing knob may be required to bring the image of
the specimen into sharp focus. Once the specimen is in focus with the
highest power objective, it will be in focus with each lower power
objective.
OIL IMMERSION (Only for models with a 100X objective)
When light rays from the objective lens to the specimen pass through air,
they are distorted slightly, a phenomenon known as refraction. This is
usually not a problem at a magnification of 400X or lower. However, at a
magnification of 1,000X and above, refraction becomes problematic.
This problem is reduced significantly by placing a thin layer of very clear,
viscous oil between the slide and tip of the objective lens. The result is a
much clearer image at 1,000X because the oil has the same light
transmitting properties as glass. Using oil slightly increases the
resolution and brightness of the image. Usually a very thin slide (size #1)
is used for oil immersion because at this magnification, the working
distance is very small and is critical to focusing the specimen. Good
quality glass (not plastic) cover slips should be used. If their thickness is
over 0.17mm, the objective will not resolve properly, because the
specimen cannot be moved close enough to the objective lens to be in
focus.
Place a tiny amount of oil (only 1 drop should be sufficient) onto the slide
prior to rotating the 100XRD objective into position. It is essential to
thoroughly clean the objective tip after use to prevent damage and to
ensure that an image can be seen clearly the next time the objective is
used. Please contact Swift Optical or your authorized Swift dealer for the
appropriate immersion oil to use.
Summary of Contents for M3500 SERIES
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