7. Quality Control
7. Quality Control
7.1 Periodic Quality Control Tests
Depending on usage and application we recommend a periodic evaluation of the
instrument on TECAN site.
The tests described in the following chapters do not replace a full evaluation by
the manufacturer or authorized dealers. But the tests maybe performed
periodically by the user to check significant aspects of the instrument
performance.
The results are strongly influenced by errors in pipetting and the setting of the
parameters in the instrument. Therefore please follow the instructions carefully.
The user should determine the appropriate intervals for this testing based on how
frequently the instrument is operated.
We recommend adapting these tests and the acceptance criteria to the
laboratory’s primary application. Ideally these tests must be performed with the
laboratory’s own plates, fluorophore, buffers, volumes and all the appropriate
settings (filters, flashes, delays, top/bottom reading, and so on).
7.2 Fluorescence
7.2.1 Sensitivity
Test
•
Use a black 96 well plate, for example: Greiner flat bottom, black microplate
•
Apply 200 µl of 1 nM Fluorescein in 0.01 M NaOH solution to wells A1 to H1.
We recommend SIGMA fluorescein F-6377 (acid yellow 73, FW 376.3)
•
All other wells are blank (
B
), for example: 200 µl 0.01 M NaOH solution
without fluorescein
B
C
D
E
F
G
H
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
1
2
3
4
5
6
7
8
9
10
11
12
A
•
Use 100 flashes and optimal gain function.
June 2002
Operating Manual for GENios, GENios FL, and GENios
Plus
No: I 112 904 Rev No: 1.1
7-1
