4. Operating Instructions
36
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
2022-01
4.7
Drip Mode
The slowest dispensing mode is drip mode.
Drip mode is used for very sensitive applications, e.g., washing cell layers,
because the instrument offers the possibility to dispense liquid in small drops.
This minimizes the detachment of cells and increases the remaining cell
percentage at the bottom of the wells.
The following example shows a typical wash program for working with adherent
cells (as it would appear in the Define/Edit menu, see 5.2.2 Define/Edit a
Program (Define/Edit Menu) for further information about defining programs).
Note
The following example program should not be used as a standard wash
procedure for cell washing as it is necessary to adjust the wash
parameters, such as Z-
position “MOVE CUST.”, dispensing and aspirating
rates, head speed, etc., according to the cell type used.
When the
Move
function is selected in combination with dispensing, the wash
head will first move down to the bottom of the wells and then upwards to the
overflow position. To minimize cell detachment in drip mode, it is recommended
to use a customized plate definition file with elevated z-position
Bottom
of approx.
7000 µm.
