7. Performance Testing/ Quality Control
54
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
2022-01
Programs Necessary for QC Procedure
Define the following programs to perform QC procedures:
QCDISP
1. Plate Mode
2. Plate Type: Greiner 96-well flat bottom
3. One Cycle
4. One Dispense step with the following parameters:
o
POS: OVERFLOW
o
VOLUME 200 µl
o
CHANNEL 1
o
DISPENSE RATE 200 µl/s
QCASP
1. Plate Mode
2. Plate Type: Greiner 96-well flat bottom
3. One Cycle
4. One Aspiration step with the following parameters:
o
Crossw. ASP
o
POS: BOTTOM
o
TIME: 4 s
o
H-SPEED 10 mm/s
o
ASP.RATE 3
Residual Volume Check
Note
Ensure that the lab balance is calibrated. Ensure that the
HYDROFLEX PLUS and the waste and liquid bottles are placed at the
same height on a vibration-free surface according to the manufacturer
’s
guidelines.
Record serial number of lab balance and the HYDROFLEX PLUS used, as well
as name of operator for documentation purposes.
1. Connect HYDROFLEX PLUS to waste bottle as described in this manual in
2. Connect wash-buffer bottle containing solution for QC-procedure to channel 1
of HYDROFLEX PLUS as described in this manual in 2.4.2 Rear Panel
Connections.
3. Prime channel 1 of the HYDROFLEX PLUS for 10 seconds as described in
4. Weigh empty and dry Greiner96ft specified above on lab-balance and record
TARE.
5. Load Greiner-F microplate on HYDROFLEX PLUS to be checked and start
program QCDISP to dispense 200µl of liquid into each well.
6. Weigh the filled microplate and record the weight. Check dispensing accuracy
visually.
7. Start program QCASP to remove the dispensed liquid out of the wells.
