8. Maintenance and Cleaning
60
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
2022-01
8.2
Priming
Priming is performed to fill the liquid system of the HYDROFLEX PLUS with liquid
and to remove all air from the tubes. A priming step must also be performed when
switching between buffers.
If different wash buffers are used in a wash program, the instrument performs a
prime step automatically before switching to the next liquid. A prime step is also
automatically performed before a program is started.
If the instrument will be left to stand for a longer time, priming must be performed
to remove all liquid from the system. For this purpose, remove all tubes from the
liquid bottles.
WARNING
BEFORE THE INSTRUMENT IS USED, ALL DISPENSING CHANNELS
NEEDED FOR THE WASH PROGRAM MUST BE PRIMED TO FILL
THE LIQUID SYSTEM WITH THE REQUIRED LIQUID.
IF THE PRIMING PROCEDURE IS NOT PERFORMED PROPERLY,
THIS CAN RESULT IN INSUFFICIENT WASHING OF THE WELLS AND
CAN SERIOUSLY AFFECT ASSAY PERFORMANCE.
ENSURE THAT THE BOTTLE OF THE PRIMING SOLUTION IS
ALWAYS FULL AT THE BEGINNING OF THE PRIMING PROCEDURE
AND CHECK THAT THE INLET FILTERS IN THE LIQUID TUBES ARE
CLEAN.
CAUTION
ENSURE THAT THE DISPENSING AND ASPIRATION PUMPS ARE NOT
RUN FOR LONGER THAN A FEW MINUTES WITHOUT LIQUID OR THEY
WILL BE DAMAGED.
CAUTION
DO NOT USE THE INSTRUMENT TO ASPIRATE OR DISPENSE ANY
ACIDIC SOLUTIONS AS THIS COULD DAMAGE THE INSTRUMENT.
