6. LSServer, Scanner Shell and Download
2004-11
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
6-79
All data needed for calculating crosstalk correction must be entered / selected in
the
Crosstalk
dialog box.
For information about how to get special data needed for crosstalk correction and
how to perform the correction, refer to the
LSServer Beginners Guide
manual
.
In the
Crosstalk
dialog box, all data needed for crosstalk correction must be set.
The requested options must be entered in the corresponding edit boxes,
selections must be made in combo and list boxes.
The
Crosstalk
dialog box is divided into four areas:
Channel 1 / Channel 2 tab:
In the
Channel 1 / Channel 2
tab, data must be entered, which has been
previously determined through special measurement (e.g. using Tecan LSA
Software for microarray analysis): Dye 1, Dye 2, K1, K2, Gain 1 and Gain 2.
Laser / Filter selection:
In the
Laser / Filter
combo box, laser and filter combinations for crosstalk must
be selected.
Current Date and Name edit box:
In the
Date
field the current date is displayed. In the
Name edit
box name or
initials must be entered
K1 / K2 data list box:
All data selected / entered in the
Crosstalk
dialog box is stored as a crosstalk
factor record (CrosstalkCor.dat). This can be added to
K1 / K2 data
list box.
Clicking on an item in the list box displays corresponding data. Crosstalk factor
records can be added, changed or deleted at any time.
Click
OK
to save all
K1 / K2
list box data in a special file
CrosstalkCor.dat
and
close the dialog box.
The first part of
Crosstalk
dialog box is divided into individual tabs:
1. Channel
1
tab
Before calculating crosstalk correction a so-called K-factor must be
determined. This is done during a measurement without taking into account
crosstalk correction. Adjusted values in this measurement session must be
entered in the
Channel 1
tab.
The
Channel 1
tab contains the following elements:
Dye 1
edit box
In the
Dye 1
edit box the description of used dye must be entered.
K1
edit box
In the
K1
edit box the correction factor for contribution of dye with
shorter emission wavelength into the detection channel of dye with
longer emission wavelength must be entered.
The
K1
default value is set to 0. This means no crosstalk correction
will be done, no other parameters (Dye, gain) must be specified.
Gain 1
edit box
In the
Gain 1
edit box PMT Gain channel 1 must be entered.
Gain 2
edit box
In the
Gain 2
edit box PMT Gain channel 2 must be entered.
