6. LSServer, Scanner Shell and Download
2004-11
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
6-17
to confirm the newly defined areas, (see figure 6-2, on page 6-15 for a picture of
the AGC area). AGC is only available in:
•
Single Scan Mode
•
Dual Sequential Scan Mode
It is not possible in Dual Parallel Scan Mode because the Cross Talk
phenomenon with special dye combinations (e.g. Cy3 and Cy5), where signal
from one channel will be detected in the other channel and can therefore lead to
incorrectly calculated gain values. To start a scan with the AGC feature first of all
a special area for AGC must be selected. This can be done from either the
Geometry Parameters
tab of the
Measurement Parameter
dialog box or during a
prescan from the
Prescan
dialog box:
Figure 6-4 Geometry Parameters
tab
Figure 6-5
Prescan dialog box
(For more information, see AGC (Automatic Gain Control) on page 6-37).
When a scan with AGC is started, the instrument performs a scan of the selected
AGC-area. After this scan, all pixels of that image will be calculated and will be
virtually drawn in a histogram, where the amount of pixels against the signal
values will be plotted.
