6. LSServer, Scanner Shell and Download
6-24
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
2004-11
Focus Prescan
In the
Measurement Control
dialog box, click
Prescan
and select
Focus
Prescan
to perform a focus scan of the first selected scan area of the currently
used MTP adapter.
The focus prescan enables the user to define the desired focal plane and the
focus offset on a z-scan image. This can also be used for probes, where the auto
focus fails. The focus offset can be adjusted directly on the image, so refraction
considerations are not necessary.
If no focus was previously selected, the focus prescan will be aborted with
following error message: ‘No focus mode selected. Please select focus mode in
Advanced Autofocus dialog!’
Fluorescence focus scans will be performed with the laser / filter setting of
channel 1. This focus scan mode can be relevant for gel applications. The default
settings are reflection modus with the red ( 633nm ) laser.
Click
OK,
and
Focus Prescan
dialog box appears:
Example of HS and well mode auto focus.
The
Focus Prescan
dialog box has the following buttons:
Reset
button
Click
Reset
to return all values to the default settings.
OK
button Click
OK
to save the settings. The focus values are stored for the
currently scanned slide. A message appears, which asks if the focus
offset should only be stored for this slide or for all slides of the
adapter.
Cancel
button
Click
Cancel
to close the dialog box.
The selected options are not saved.
Help
button
Click
Help
to display the online help for the dialog box.
