6. LSServer, Scanner Shell and Download
2004-11
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
6-47
Autofocus Selection
Autofocus
Selection
group box
•
No Autofocus
option button
Select
No Autofocus
option button, if no autofocus measurement is
required.
The autofocus scan prior to the measurement is skipped and the
measurement is performed at the plane that is calculated from the
Adapter height, Slide Thickness and Focus Offsets.
The scan level is calculated by considering the default height of the
Tecan MTP Slide Adapter
(= 11,5 mm)
, the slide thickness, as well as
the Focus Offset.
No Autofocus
eliminates the green autofocus scan lines from the
Geometry Parameter tab.
The
No Autofocus
option is recommended to use in combination with a
medium or large pinhole.
•
HS Autofocus
option button:
Select
HS Autofocus
option button to perform the so-called five point
autofocus.
To determine the right focal plane, the plate transport is moved in vertical
direction (z-direction) creating a signal intensity profile from the laser
reflection at the surface of the samples. From the 5 detection points a
focal plane will be determined.
•
Well Mode
option button:
Select the
Well Mode
option button to perform a two point autofocus prior
to measurement.
The autofocus in
Well Mode
is appropriate for microplates and all similar
formats, where due to wells or other reasons a continuous focal plane is
missing. The algorithm considers only the edge values in the focus.tif
image.
•
MTP Mode
option button:
Select
MTP Mode
option button to perform a triangle autofocus prior to
measurement.
The autofocus in
MTP Mode
performs three z-scans (Focus1, 2 and 3)
on three positions in a triangle order onto a microplate. Between these
three points the overall focal area is calculated.
