tecan Te-VacS, Operating Manual

Page 1: ...Operating Manual Tecan Vacuum Separator Te VacS Tecan Vacuum Separator Te VacS Operating Manual 391236 en V4 0...

Page 2: ...hanced options 3 0 2009 03 20 Changes Updated software and hardware refer ences added foamy solutions info added spacer guide updated pictures updated spare part list and numbers various minor changes...

Page 3: ...Application Software Manuals 8 1 8 Compliance with Laws and Standards 8 1 9 Document Conventions 10 2 Safety 11 2 1 Safety Message Conventions 11 2 1 1 Signal Words 11 2 1 2 Safety Symbols 11 2 2 Gene...

Page 4: ...Waste Bottle 21 4 3 Base Plate Vacuum Block 22 4 4 Vacuum Block 22 4 5 Spacers 22 4 6 Optional Accessories 22 4 7 Operating Elements and Displays 22 4 8 Function 23 4 8 1 Basic Principle of an Extrac...

Page 5: ...Agents 38 6 3 Maintenance Schedule 39 6 3 1 End of Day Maintenance Table 39 6 3 2 Monthly Maintenance Table 40 6 4 Maintenance Tasks 41 6 4 1 Cleaning the Te VacS 41 7 Troubleshooting 42 7 1 Troublesh...

Page 6: ...Table of Contents 6 54 Tecan Vacuum Separator Te VacS Operating Manual 391236 en V4 0...

Page 7: ...S 1 2 Manufacturer Address of Manufacturer Tecan Schweiz AG Seestrasse 103 CH 8708 M nnedorf Switzerland 1 3 Intended Use The Te VacS is used for rapid separation or purification of biological molecul...

Page 8: ...ing the Te VacS The Doc IDs listed below are root numbers Therefore they do not contain information about the language document version or the medium data storage medium hard copy downloadable file et...

Page 9: ...Manual Compliance with Laws and Standards Tecan Vacuum Separator Te VacS Operating Manual 391236 en V4 0 9 54 Refer to the corresponding Operating Manual of the instrument Refer to section Reference...

Page 10: ...ss references appear as follows e g Refer to section Safety 11 Safety refers to the corresponding section header The page number is given in square brackets Prerequisites Prerequisites appear as follo...

Page 11: ...al Words Signal Word Meaning Indicates a hazardous situation that if not avoided will result in death or serious injury Indicates a hazardous situation that if not avoided could result in death or ser...

Page 12: ...dified in any way without prior consultation and written approval of Tecan Authorized modifications to the system may only be performed by an FSE certified for the repair and upgrading of the Te VacS...

Page 13: ...operate the Te VacS The work described in this Operating Manual must only be performed by authorized personnel with the qualifications prescribed below Laboratory personnel must have suitable technic...

Page 14: ...he Te VacS In the event of accidents e g crash spillage etc Before returning the Te VacS or its parts or accessories to Tecan e g for repair Prior to storage Prior to disposal In general before moving...

Page 15: ...type plate is on the rear side of the Te VacS and contains the following information Identification data Model REF Ordering information material number and revi sion level Date of manufacture YYYYMMDD...

Page 16: ...Width w x Height h Te VacS tower 300 mm x 700 mm x 340 mm 11 8 in x 27 6 in x 13 4 in Waste bottle 160 mm x 160 mm x 530 mm 6 3 in x 6 3 in x 20 9 in Base plate vacuum block 285 mm x 155 mm x 49 mm 11...

Page 17: ...bar 3 5 Environmental Conditions Te VacS is intended for indoor operation and storage only For detailed information on operating conditions refer to the Operating Manual of the instrument the Te VacS...

Page 18: ...e aware that chemical resistance varies with temperature and solvent concentration 3 6 1 Chemical Resistance to Liquids 1 resistant 2 resistant to a limited extend 3 not resistant Parts DMSO Acetonitr...

Page 19: ...3 Foam Formation Substances causing foam formation should be avoided Please note that often protein solutions can be a source of foam If foam formation cannot be avoided it is recommended to install a...

Page 20: ...ription of the assemblies 4 1 Overview The Te VacS cannot be operated as a stand alone system but only as a device integrated into a Tecan instrument The microplates SPE cartridge blocks and the vacuu...

Page 21: ...he system control and controls the vacuum pump accordingly The interface of the board to the instrument is given by D sub plug 9 pin male on the front side of the control unit 4 2 2 Vacuum Unit The va...

Page 22: ...e SPE cartridge block The abrupt ventilation of the vacuum block causes turbulences on the underside of the wash adapter that tear off dangling drops 4 5 Spacers The spacer is used to decrease the dis...

Page 23: ...an be applied to the base plate vacuum block I This results in drainage of the liquid from the SPE cartridge block into the waste bottle The vacuum can be released via ventilation valves H To compensa...

Page 24: ...collected either in a 96 well microplate or in a deepwell plate 4 8 1 Basic Principle of an Extraction Process Illustration 8 Basic principle of an extraction process A SPE cartridge block B Single we...

Page 25: ...h different time dependencies of the pressure drop in the vacuum block are used Fast pumping Soft pumping Fast Pumping With the fast pumping procedure at first the extraction valves are closed while t...

Page 26: ...ation 10 Pressure drop in the vacuum box during soft pumping Reaction time in the separation columns t0 Start of pumping the waste bottle The soft pumping procedure is applied if slow processing is de...

Page 27: ...provides reliable and reproducible performance Ensure that the validation process is executed according to national laws and standards 5 2 Putting into Operation 5 2 1 Mounting the Base Plate Vacuum...

Page 28: ...ttle To prepare the waste bottle proceed as follows 1 Remove the waste bottle cap A A B Illustration 13 Preparing waste bottle 2 Ensure that the waste bottle is empty 3 Ensure that the sealing B is pr...

Page 29: ...tubing into five pieces that are specified as follows 3 pieces with the same length for the connection between the waste bottle and the vacuum unit A tube length of 65 cm 26 in is recommended 2 piece...

Page 30: ...tle proceed as follows 1 Connect the waste bottle to the Te VacS tower using three tubes of the same length A B C A B C D A B D C Illustration 16 Waste bottle connections 2 Connect the liquid level se...

Page 31: ...e Te VacS tower using two tubes of the same length A B A B C E A D E B C D Illustration 17 Waste bottle connections 2 Connect the two waste bottles using a tube that is at least 5 m long C 3 Connect t...

Page 32: ...ng connections at the Te VacS tower A B using two tubes of the same length A D B C C D A B Illustration 18 Vacuum block connections 2 Connect the ventilation holes of the base plate vacuum block with...

Page 33: ...y after disassembly e g for cleaning or moving purposes The color markers are delivered with the Te VacS 1 After all tubes are correctly connected to the Te VacS tower label each connection with a col...

Page 34: ...2 8 Mounting the Sound Absorber 1 Remove the fixing nut of the tubing connector at the exhaust outlet C of the vacuum unit A 2 Screw on the sound absorber B to the exhaust outlet of the vacuum unit A...

Page 35: ...nge the sheet metal frame on your vacuum block with the appropriate one that was delivered with your vacuum block For exchanging the sheet metal frame of the vacuum block proceed as follows 1 Remove t...

Page 36: ...propriate spacer should be used Spacer selection depends on the SPE cartridge block type and the height of the collection plate To select the appropriate spacer proceed as follows 1 Measure the distan...

Page 37: ...the closest lower value in the table Spacer Height mm Spacer No Tecan Part No 7 5 1 10760663 26 5 2 10760662 3 5 3 10760661 33 5 4 10760652 16 5 5 10760664 36 2 6 10760666 10 5 7 10760691 Table 4 Avai...

Page 38: ...method must be defined by the key operator based on the type of contaminant and degree of contamination Guidance on the selection of decontamination agents and application modes is provided in this c...

Page 39: ...ntenance Task Cleaning Agent Reference Base plate vac uum block Spacer Vacuum block Clean and decon taminate Use isopropyl al cohol a commer cially available mild detergent or a decontamination soluti...

Page 40: ...omponent Maintenance Task Cleaning Agent Reference Maintenance Activi ties Base plate vac uum block Check the sealing Silicone grease If the sealing sticks to the microplate clean and lubricate the se...

Page 41: ...Tasks 6 4 1 Cleaning the Te VacS 1 Clean the base plate vacuum block the vacuum block the spacer if used the waste bottle and the tubes 2 Clean the ventilation holes A and extraction holes B in the b...

Page 42: ...pressure Performance test failed Check the tubing the tubing con nectors and the sealings If the problem cannot be solved please consult the Customer Sup port 51 Valve vacuum pump or pressure sensor...

Page 43: ...r ing carriers block the arm ac cess Vector setpoints for the arm incorrect Check the positioning pins Check the distance to the neigh boring carrier on the worktable Check the vector setpoints for th...

Page 44: ...g accidents incorrect delivery loss of weight and damage during storage Symbol Meaning Description Recycle The packaging material can be recycled Do not dispose of as domestic waste Information on the...

Page 45: ...perating Manual 391236 en V4 0 45 54 8 2 Disposal NOTICE Recycling in accordance with applicable legal regulations Observe the laws applicable in your country for recycling For further information ref...

Page 46: ...when ordering spare parts This chapter only contains spare parts that can be replaced by the operator To order spare parts other than listed here please consult the Customer Support 51 9 1 Te VacS To...

Page 47: ...tain any liquid level sensor This bottle is used for foamy solutions only 30045220 BOTTLE WASTE 4LWITH CAP Table 10 Spare Parts for the waste bottle 9 3 Tubing and Cables No Plain Text Designa tion SA...

Page 48: ...signation 1 Spacer no 1 height 7 5 mm 10760663 SPACER NO 1 H7 5MM SPE TE VACS 2 Spacer no 2 height 26 5 mm 10760662 SPACER NO 2 H26 5MM SPE TE VACS 3 Spacer no 3 height 3 5 mm 10760661 SPACER NO 3 H3...

Page 49: ...760637 10760624 BLOCK VACUUM PP H37MM TE VACS TYPE A 2 SPE Vacuum block type B short 37mm high Material PMMA non chemical resis tant transparent For bottom skirted car tridges from Eppen dorf Invitrog...

Page 50: ...uum box type P ALU for TecPrep96 plates designed for spotting onto MALDI targets with reposi tioner 10760902 BOX VACUUM ALU TYPE P TE VACS 6 SPE vacuum box type PW ALU for TecPrep96 plates wash design...

Page 51: ...page of the help file context sensitive help of software products 10 1 Contacts Please contact your local distributor or one of the addresses below Also see our homepage on the web www tecan com Count...

Page 52: ...y Phone Fax E mail 49 1805 8322 633 or 49 1805 TECAN DE 49 7951 9417 92 helpdesk de tecan com Italy Tecan Italia S r l Via Brescia 39 20063 Cernusco Sul Naviglio MI Italy Phone Fax E mail 39 800 11 22...

Page 53: ...22 89 23 helpdesk ch tecan com United King dom Tecan UK Ltd Theale Court 11 13 High Street Theale Reading RG7 5AH United Kingdom Phone Fax E mail 44 118 930 0300 44 118 930 5671 helpdesk uk tecan com...

Page 54: ...System FSE Field Service Engineer IEC International Electrotechnical Com mission IQ installation qualification ISO International Organization for Stan dardization MALDI Matrix Assisted Laser Desorpti...