CSB CryoEM Facility TF20 Operating Instructions SEC
05/02/16
VII.
End of Session
14.
Close column valves
15. Retract the campera
16. Put the screen down
17.
Home the stage (go to the Stage tab Control and click on Holder, this ensures that the stage
is at 0um in X, Y and Z.
18.
Remove the holder carefully.
19.
Click on cryo cycle. Make sure the cryo cycle will run for 365 minutes or more.
Wait ~5 minutes or for Turbo to turn yellow before removing the liquid nitrogen dewar
20.
Remove the dewar from the microscope and dispose of the remaining LN2 in the foam
container under the table.
21.
Place the protective fabric underneath the anticontaminator.
22.
Dismount the specimen from the holder and dispose of it (or save it) properly.
23.
If using the RT or RT tomography holders, return them to their proper receptacle.
24.
If using the Gatan cryo holder(s), return to the dry pumping station and follow the instructions
there on how to warm it and pump it properly.
25.
Close Digital Micrograph and the Tecnai User Interface and log out.
26.
Please sign up on the user's sheet. Record the actual time spent imaging.
27. Return the next day to retrieve Data. Data will be moved overnight to the computer located
behind the curtain in the F20 room