Chapter 5
Operation
55
Figure 5-4: Typical WinX/32 Experiment Setup
►
Timing Tab
Acquiring Data
1. If you are using WinView/32 and the computer monitor for focusing, select Focus
from the Acquisition menu. Successive images will be sent to the monitor as quickly
as they are acquired.
2. Adjust the lens aperture, intensity scaling, and focus for the best image as viewed on
the computer monitor. Some imaging tips follow:
•
Begin with the lens blocked off and then set the lens at the smallest possible
aperture (largest f-stop number).
•
Make sure there is a suitable target in front of the lens. An object with text or
graphics works best.
•
Adjust the intensity scaling (by clicking the 5%-95% button at the bottom left
corner of the data window) and adjust the lens aperture until a suitable setting is
found. Once you've determined that the image is present, select a lower setting
for better contrast. Check the brightest regions of the image to determine if the
A/D converter is at full-scale. A 16-bit A/D is at full scale when the brightest
parts of the image reach an intensity of 65535. Adjust the aperture to where it is
just slightly smaller (higher f-stop) than the setting where maximum brightness
on any part of the image occurs.
•
Set the focus adjustment of the lens for maximum sharpness in the viewed
image.
3. After you have focused the camera, you can stop Focus mode, continue Focus
mode, begin Acquire mode, or wait for the CCD to reach the operating temperature
before going to Acquire mode.
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Summary of Contents for ProEM Series
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