background image

Figure 6   Custom Methods screen options

Select a pre-programmed method

1.

On the 

Home

 screen touch 

Pre-programmed Methods

.

2.

In the 

Pre-programmed Methods

 screen, touch either 

Mini gels

Midi gels

, or

Western blots

 (see Figure 7).

• Selecting Mini gels brings up a list of preloaded methods for

electrophoresing mini gels sold by Thermo Fisher Scientific, Inc.

• Selecting Midi gels brings up a list of preloaded methods for

electrophoresing midi gels sold by Thermo Fisher Scientific, Inc.

• Selecting Western blots brings up a list of preloaded methods for

transferring above gels to Western blots

3.

To view method options not available on the first screen, gently swipe up with
your finger as you would with a smart phone.

4.

Touch a method to bring up the 

Run

 screen for that method.

These screens display default running conditions (voltage, and time) for the
pre-programmed method selected. The current and power settings shown are
the power supply maximums.

Chapter 2 

Methods

Select a pre-programmed method

2

14

PowerEase

 Touch 350W Power Supply User Guide

Summary of Contents for Invitrogen PowerEase Touch PS0350

Page 1: ...Use Only Not for use in diagnostic procedures PowerEase Touch 350W Power Supply Catalog Numbers PS0350 115 VAC PS0351 230 VAC PS0352 230 VAC Australia PS0353 230 VAC China Publication Number MAN0019503 Revision A 0 ...

Page 2: ...t 2020 New document Important Licensing Information These products may be covered by one or more Limited Use Label Licenses By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses NOTICE TO PURCHASER DISCLAIMER OF LICENSE Purchase of this software product alone does not imply any license under any process instrument or other apparatus system compos...

Page 3: ...onstant Power 13 Custom methods 13 Select a pre programmed method 14 Manage methods 16 Save a method 16 Edit a method 17 Delete a method 18 Change the name of a saved method 18 Add a step 19 Delete a step 19 Save a program 19 Instrument display during a run 20 Run screen features 20 Method Settings screen features 21 Instrument display after run completion 22 Settings 22 Settings screen features 2...

Page 4: ...strument safety 31 Avoid electrical shock 31 Avoid damage to the instrument 31 Explanation of symbols and warnings 32 Symbols and warnings 32 Product regulatory compliance 33 Federal Communications Commission Advisory 33 Environmental design compliance 33 APPENDIX E Product specifications 34 Product specifications 34 APPENDIX F Documentation and support 36 Customer and technical support 36 Limited...

Page 5: ...Sturdy polyurethane feet and stackable housing design allow stacking of power supplies Running modes include Constant Voltage Constant Power or Constant Current Programming capabilities for limiting voltage V current mA and power W Method programming flexibility up to 100 methods 20 steps per method 999 minutes per step Pause button for editing method parameters during run User adjustable device s...

Page 6: ...ver Rear view of PowerEase Touch 350W Power Supply The USB port is shipped with a plastic dust cover for protection during shipment This cover must be removed prior to port use Remove by gently prying off with a small flat blade screwdriver Chapter 1 Product information Product description 1 6 PowerEase Touch 350W Power Supply User Guide ...

Page 7: ...w Zealand customers should use the Type I power cord Table 1 Cat No PS0350 115 VAC Component Quantity Regulatory mark s on electrical plug PowerEase Touch 350W Power Supply 1 each Extra Fuse 1 each Type B Power Cord 1 each CSA JIS 8303 Power Cord 1 each PSE USB Cover 1 each Quick Reference Card 1 each Certificate of Performance 1 each Table 2 Cat No PS0351 230 VAC Component Quantity Regulatory mar...

Page 8: ...rence Card 1 each Certificate of Performance 1 each Table 4 Cat No PS0353 230 VAC China Component Quantity Regulatory mark s on electrical plug PowerEase Touch 350W Power Supply 1 each Extra Fuse 1 each Type GB 15934 2008 Power Cord 1 each CCC USB Cover 1 each Quick Reference Card 1 each Certificate of Performance 1 each Chapter 1 Product information Product contents 1 8 PowerEase Touch 350W Power...

Page 9: ...lectrophoresis product we recommend that you adhere to the protocols given in the electrophoresis product manuals For best results We recommend using the Constant Voltage Mode for protein gels or blots Note For most electrophoresis methods resistance increases throughout the run Limiting the voltage allows for an improving margin of safety over time because the current and power decrease through t...

Page 10: ... to the power supply by inserting the positive lead into the red output jack and the negative lead into the black output jack 7 Turn the AC power switch at the back of the power supply On Programming options The PowerEase Touch 350W Power Supply can be programmed to run single step and multi step methods Up to 100 user defined methods can be saved up to 20 steps can be saved for each multi step me...

Page 11: ... will appear on the Home screen when the power supply is switched on for the first time or it if is switched on after running an unsaved custom method see below The Last run option will appear on the Home screen when the power supply is switched on after having previously run a saved custom method or a pre programmed method It will also be shown after an un saved custom method is run until the pow...

Page 12: ...creen to bring up the Run parameter edit screen Default maximum values are 300 V 3 000 mA and 350 W Note When using constant voltage or constant power it is recommended that the maximum default settings not be changed to avoid unwanted power limitations during the run 5 Enter the parameter value required then touch Enter to advance with the newly changed run parameter displayed 6 Optional Add or d...

Page 13: ...e may cause the voltage to increase to the maximum capacity of the power supply This may lead to local overheating and damage to the electrophoresis cell or create unsafe conditions Note When operating under constant current conditions it is recommended to set a voltage limit on the power supply slightly above the maximum expected for the run Constant Power Power is a function of voltage and curre...

Page 14: ...phoresing midi gels sold by Thermo Fisher Scientific Inc Selecting Western blots brings up a list of preloaded methods for transferring above gels to Western blots 3 To view method options not available on the first screen gently swipe up with your finger as you would with a smart phone 4 Touch a method to bring up the Run screen for that method These screens display default running conditions vol...

Page 15: ...or your gel may be slightly different than the default value given by the pre programmed method The default run times shown in mini gel pre programmed methods assume the use of the Mini Gel Tank Thermo Scientific Cat No A25977 Midi gel run times assume the use of the SureLock Tandem Midi Gel Tank Thermo Scientific Cat No STM1001 Figure 7 Pre programmed Methods Screens For example The pre programme...

Page 16: ...dited pre programmed method by touching Edit 3 Touch the Name of method field in the Save As screen to bring up a keyboard that will allow entry of the method name Method names can contain a maximum of 14 characters Keyboards displaying numbers and lower case letters are accessed using buttons in the lower left corner of the screen 4 Enter the name touch Enter then touch Save Note Do not save cust...

Page 17: ...hod touch the data field for the parameter you wish to change Selecting a run parameter will bring up its Edit screen 2 Enter the parameter value required then press Enter Note Do not save custom methods with the same name as a pre programmed method Chapter 2 Methods Manage methods 2 PowerEase Touch 350W Power Supply User Guide 17 ...

Page 18: ...list of displayed methods then press Delete If several methods have been saved swipe up as you would with a smart phone to scroll down the list Figure 10 Managing saved methods Change the name of a saved method 1 Touch Actions then Save As see Figure 9 This will bring up the Save As screen with the current method name as the header 2 Touch the method name and use the keyboard to change the name 3 ...

Page 19: ... previous step or right arrow next step 3 When all of the steps have been entered touch Start run to initiate the run To previous step To next step Figure 11 Method step number location Delete a step 1 Navigate to the step to be deleted using the left and right arrows on the Constant parameter screen see Figure 11 2 Touch Actions then Delete step Save a program 1 Touch Actions then Save As 2 Touch...

Page 20: ...ings screen Run screen features Method name The name of the method will be displayed at the top of the screen If the method is an un named custom method the name displayed will be the method type Constant Voltage Constant Current or Constant Power The method displayed in Figure 12 is a custom constant voltage method Center dial The center dial displays Countdown timer in hours minutes and seconds ...

Page 21: ...ack to the set method constant parameter 200 V in this case and the crossover icon will no longer be displayed Figure 12 shows a run with crossover to A and current limited to 0 04 A For additional details see Appendix A Troubleshooting Stop and Pause buttons The run can be stopped or temporarily paused by touching Stop or Pause The method can be edited when paused Right navigation arrow Touch thi...

Page 22: ...t be displayed Screen for non saved custom methods Screen for saved or pre programmed methods Figure 13 Run complete screens Settings The PowerEase Touch 350W Power Supply is equipped with a number of user adjustable settings see Figure 14 The settings menu is accessed from the Home screen by touching the Settings icon see Menu navigation on page 11 To view settings options not available on the fi...

Page 23: ...htness Allows adjustment of screen brightness Press to increase brightness or to reduce brightness Volume Allows adjustment of beeper volume Press to increase volume or to reduce volume Sleep mode Allows adjustment of the inactivity time before the screen enters sleep mode screen dark The default sleep mode setting is Enable and set to 2 minutes see Figure 15 1 To turn sleep mode off move the slid...

Page 24: ... FAT32 format and have a maximum capacity of 32 GB 2 On the Settings screen touch Software update see Figure 14 The power supply will search for update files on the USB drive This will take several seconds 3 When the updated software has been found touch Update on the displayed screen The update may take a couple of minutes to install 4 After software installation the power supply will automatical...

Page 25: ... buffer levels then press Restart to restart the run Operation stops with alarm Display shows Over Voltage Output voltage was too high and the power supply stops the output Verify cable connections Verify that the gel and buffers are correct Press Restart to restart the run Operation stops with alarm Display shows Ground Leak Ground leak detected during run Check the electrophoresis system for imp...

Page 26: ... run Excessive buffer evaporation Ensure electrophoresis settings and buffer concentration is correct Ensure wire connections are intact Press Resume to continue the run or Restart to restart the run Loose connection in a connected system Ensure cable connections are intact Press Resume to continue the run or Restart to restart the run Change in constant mode Crossover Current set too low Current ...

Page 27: ...al fuses contact support see Appendix F Documentation and support 1 Turn off the power switch and detach the power cord at the rear of the power supply unit 2 Open the fuse compartment located inside the power inlet by inserting a small flat blade screwdriver into the slot on top of the fuse compartment Turn the screwdriver to gently pry open the fuse compartment Note The fuse compartment will not...

Page 28: ...ap the cover closed and re attach the power cord Figure 16 Rearview of PowerEase Touch 350W Power Supply Appendix B Repair and maintenance Replace the fuse B 28 PowerEase Touch 350W Power Supply User Guide ...

Page 29: ...ving highly conductive chloride ions in the gel are gradually replaced by the slower moving less conductive glycine ions from the running buffer as the gel runs As a result the resistance of the gel increases as the chloride glycine front moves down the gel Voltage The velocity with which an ion moves in an electric field will vary in proportion to the field strength volts per unit distance betwee...

Page 30: ...r in watts or the rate of heat generated by the system is directly proportional to voltage and current P IV Appendix C Electrical parameters for electrophoresis Power C 30 PowerEase Touch 350W Power Supply User Guide ...

Page 31: ...d on the screen WAIT at least 5 seconds after stopping a run before handling output leads or connected apparatus ALWAYS make sure that hands work area and instruments are clean and dry before making any connections or operating the power supply ALWAYS connect the power supply to a properly grounded AC outlet Avoid damage to the instrument For proper ventilation leave at least 10 cm of space behind...

Page 32: ...Pour minimiser les consequences négatives sur l environnement àla suite de l élimination de déchets électroniques ne pas éliminer ce déchet électronique avec les déchets usuels non soumis au tri sélectif Se conformer aux ordonnances locales sur les déchets municipaux pour les dispositions d élimination et communiquer avec le service à la clientèle pour des renseignements sur les options d éliminat...

Page 33: ...operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their ...

Page 34: ... voltage Operating Modes Constant Voltage Constant Current Constant Power 1 V step 1 mA step 1 watt step Timer Up to 999 min per step Crossover Automatic Display type Backlit TFT LCD Graphic type Display area 95 mm x 54 mm W x H 4 3 inch Display colors 16 7M colors Pause function Yes Safety features No Load Detection Load Change Detection Overload Detection Ground Leak Detection Programmable Yes S...

Page 35: ... x D x H Operating temperature 0 C 40 C Environmental conditions 95 RH non condensing 75 KPa 106 KPa altitude not to exceed 2 000 meters Weight 2 4 kg Certifications CE cTUVus RCM China RoHs Warranty 1 year Appendix E Product specifications Product specifications E PowerEase Touch 350W Power Supply User Guide 35 ...

Page 36: ...d protocols Certificates of Analysis Safety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Technologies Corporation and or its affiliate s warrant their products as set forth in the Life Technologies General Terms and Conditions of Sale at www thermofisher com us en home global terms and ...

Page 37: ......

Page 38: ...werEase Touch 350W Power Supply v1 GUID 97165932 C6F7 4363 8D0E 53E5A1E5A8FA 2020 08 11 05 16 19 en 22 43 45 57 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 28 August 2020 ...

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