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Installing the Nicolet iN5 Microscope 

This document contains confidential or proprietary  informat ion of Thermo  Fisher Sc ientific. Ne ither this document nor 

the information therein  is to be reproduced, distributed, used or disclosed, either  in whole o r  in part, e xcept as 

specifically authorized by Thermo Fisher Scientific. 

 

 

1/4/2018 

 

 

 

 

 

Page 21 of 84 

 

10. Connect the iN5. 

 

a.  Slide the purge sleeve back toward the iN5. 

 

 

 

b.  Set the interface latch. 

Using a 5/16 inch T-handle or L-wrench, turn the 

screw in the interface bracket completely clockwise to extend the latch and 
then turn completely counter-clock-wise to retract the latch assuring it is set 
to engage with the locator pin under iN5 baseplate.

 

 

            

  

 

                                          Extend                                                      Retract 

Summary of Contents for 840-287100

Page 1: ...mera Functional description Point and Shoot Infrared Microscope compatible w ith a Nicolet iS10 or iS50 Problems associated with this assembly Alignment Detector evacuation Optical contamination Wear...

Page 2: ...s specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 2 of 84 Rev Notes 00 Release 01 Made corrections per customer audit 02 Updated note in step 27 to test aperture position repeatabili...

Page 3: ...as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 3 of 84 Important Prior to performing this procedure you must read the Nicolet iN5 Microscope Site and Safety Guide If you do not...

Page 4: ...pectrometer a Autotune the spectrometer and ensure it passes the Performance Test and or ValPro as applicable b If the spectrometer is a Nicolet iS50 ensure firmware 1 15 or greater is installed on th...

Page 5: ...e reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 5 of 84 c Remove the secondary tray The secondary tray...

Page 6: ...pecifically authorized by Thermo Fisher Scientific 1 4 2018 Page 6 of 84 f Remove the white foam block from the top of the iN5 and set aside g Carefully lift the iN5 from the lower tray and set on a f...

Page 7: ...noc trinocular dovemount shown below and the blue tape and red cap from purge extension tube not shown and discard c Check the iN5 for damage If damage is apparent stop here and contact the factory ot...

Page 8: ...sed or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 8 of 84 b Lift straight up on the accessory to remove it from the iS10 and set t...

Page 9: ...ted used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 9 of 84 e Using the Phillips tip loosen the 2 captive cover screws on the f...

Page 10: ...the iS10 a Remove the interface bracket with screws from the plastic bag b Remove the tape from the arm of the bracket to release the bag of 4 mounting screws ensure that the locator pin does not sti...

Page 11: ...in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 11 of 84 e Slide the interface toward the iS10 until the end of the channel is fully engaged with the locator pin f...

Page 12: ...s specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 12 of 84 Remove plug from main bench at location shown g Secure the interface Using a 3 16 inch balldriver and the mounting screws s...

Page 13: ...disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 13 of 84 Install screw in main bench at location shown h If installing a new iS10 moto...

Page 14: ...roduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 14 of 84 8 Prepare the Nicolet iS50 a Release the iS50 samp...

Page 15: ...produced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 15 of 84 c Using a medium flat blade screw driver remove t...

Page 16: ...ver loosen the four latches on the iS50 main cover lift straight up to remove the cover and set the cover aside Loosen four latches and remove the cover 9 Unpack the iN5 interface bracket and connect...

Page 17: ...or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 17 of 84 c Carefully open the bag of mounting screws and set the screws aside d Conn...

Page 18: ...in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 18 of 84 f Slide the interface toward the iS50 until the end of the channel is fully engaged with the l...

Page 19: ...closed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 19 of 84 h Using a 5 32 inch balldriver remove the two purge screws from the iS50 at the f...

Page 20: ...isher Scientific 1 4 2018 Page 20 of 84 i Secure the interface Using a 5 32 inch balldriver and the mounting screws set aside in step 9c loosely thread the 2 screws into the locations shown below and...

Page 21: ...part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 21 of 84 10 Connect the iN5 a Slide the purge sleeve back toward the iN5 b Set the interface latch Using a 5 16 inch T...

Page 22: ...d either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 22 of 84 c Slide the iN5 up to the iS10 or iS50 interface bracket so the holes in the left side...

Page 23: ...until it stops The latch should engage with the locator pin under the iN5 and lock it into position If not reset the latch and then reconnect Note When first turning the screw the iN5 may appear to p...

Page 24: ...ole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 24 of 84 f Slide the purge sleeve to the left so it securely engages with the rubber gasket around the hole o...

Page 25: ...ra eyepieces X Y stage condenser system cabling iN5 Help and Microview software thermos and funnel Properly dispose of the packing materials and set all accessory cases aside 13 If this is an aftermar...

Page 26: ...Fisher Scientific 1 4 2018 Page 26 of 84 14 Prepare the detector a If the iN5 is connected to an iS50 spectrometer change the detector switch settings the iN5 is tested on an iS10 at the factory and t...

Page 27: ...Fisher Scientific 1 4 2018 Page 27 of 84 15 Install the iN5 components a Insert a 3 32 inch balldriver into the set screw on the right side of the viewer dovemount and turn counter clockwise to back...

Page 28: ...cifically authorized by Thermo Fisher Scientific 1 4 2018 Page 28 of 84 c Install the eyepieces if applicable otherwise continue with step 15d The eyepiece with reticle should be inserted in the right...

Page 29: ...d distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 29 of 84 ii Insert the condenser into the condenser rack from the...

Page 30: ...and then slide the condenser towards the thumbscrew until it stops and hold the condenser securely Then gently push the thumbscrew into threaded hole to engage the condenser and then snug the screw so...

Page 31: ...ntific 1 4 2018 Page 31 of 84 f Install the X Y stage i Using the z stage focus knobs turn the knobs clockwise away from you to lower the z stage so the z stage dovemount is just above the condenser i...

Page 32: ...uced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 32 of 84 iii Rotate the stage as necessary to square it to the...

Page 33: ...ge 33 of 84 v If you have an iN5 viewer with eyepieces insert a gold or aluminum slide into the sample holder then continue with step 16 If you have an iN5 viewer with a camera only continue with step...

Page 34: ...must be connected to the iS50 OBC right side accessory port the side the iN5 is connected to Note The photo below shows a USB cable connected to the I O panel You will not receive a USB cable unless...

Page 35: ...ole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 35 of 84 17 Turn the iS10 power supply or iS50 ON 18 Turn the iN5 power ON 19 Open MicroView and configure th...

Page 36: ...disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 36 of 84 c From the Microscope drop down menu select System Configuration and if neces...

Page 37: ...iN5 viewer with eyepieces switch the iN5 to reflection mode by moving the knob on the left side of the iN5 If you have an iN5 viewer with a camera only switch the iN5 to transmission mode by moving t...

Page 38: ...uminum slide into focus adjust the illumination as necessary If you cannot focus on the clean gold or aluminum slide move stage Y axis back so the edge of the slide is centered under the objective the...

Page 39: ...s specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 39 of 84 d While looking through the right eyepiece with your right eye rotate the entire right eyepiece to orient the cross hairs a...

Page 40: ...h the eyepieces turn the condenser knob on the lower left side of the iN5 approximately 1 turn counter clockwise to raise the condenser the field of view FOV should be illuminated b Fine tune the cond...

Page 41: ...er in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 41 of 84 c Using two 5 64 inch balldrivers adjust the condenser X Y alignment screws to center the ta...

Page 42: ...on therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 42 of 84 Using two 5 64 inch balldri...

Page 43: ...ally authorized by Thermo Fisher Scientific 1 4 2018 Page 43 of 84 26 If a camera only is installed focus the condenser using MicroView a While looking at the video screen in MicroView turn the conden...

Page 44: ...nser and adjust X Y position Ensure the condenser is focused by fine tuning the condenser knob if necessary using the dark corner corners as a reference If necessary using two 5 64 inch balldrivers mo...

Page 45: ...of the slot is flexible so the aperture can easily be inserted and removed The upper plate includes a semi circular ridge that correctly positions the aperture when the aperture is installed it should...

Page 46: ...era viewer options the sample aperture image should be centered on the red crosshair if not the aperture center will be defined using Video Calibration later in this procedure Sample aperture centered...

Page 47: ...ifically authorized by Thermo Fisher Scientific 1 4 2018 Page 47 of 84 Note Only the default files that correspond to the detector installed will be available on a new system 29 Tune the input mirror...

Page 48: ...r Scientific 1 4 2018 Page 48 of 84 c Using a 5 64 inch balldriver adjust the two opposing screws on the input mirror for best MAX interferogram peak MCT should be 7 volts MAX DTGS should be 2 5 volts...

Page 49: ...ion therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 49 of 84 If you have an iS50 spectr...

Page 50: ...isclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 50 of 84 g While viewing the interferogram change the Gain DAC 1X as appropriate to incr...

Page 51: ...used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 51 of 84 b Insert a gold or aluminum slide in the sample holder and focus on t...

Page 52: ...ly authorized by Thermo Fisher Scientific 1 4 2018 Page 52 of 84 c Using a 5 64 inch balldriver adjust the two opposing screws on the sluice mirror for best MAX interferogram peak MCT MAX peak should...

Page 53: ...observing the interferogram in Experiment Setup reinstall the main cover Lift the cover up so the points of the cover shown below are level with the left side of the frame and then turn the center sc...

Page 54: ...ic 1 4 2018 Page 54 of 84 37 Remove the 100 m fixed sample aperture and set aside 38 Reinstall the X Y manual stage and sample holder 39 Adjust the binocular viewer If a camera only is installed conti...

Page 55: ...with step 45 ii If the scale is level and you have a camera installed continue with step 40 iii If the scale is not level complete step 39e and then continue as instructed in either step i or ii abov...

Page 56: ...cifically authorized by Thermo Fisher Scientific 1 4 2018 Page 56 of 84 40 Adjust the camera rotation a If not already in reflection mode switch the microscope to reflection mode Reflection mode b If...

Page 57: ...l continue with step 41 ii If the scale is not level tilted complete step 40e and then continue with step 41 e Use a 3mm balldriver to loosen the camera mount setscrew circled below and then rotate th...

Page 58: ...isher Scientific 1 4 2018 Page 58 of 84 41 Focus the camera While viewing the calibration scale in Microview turn the camera focus ring clockwise or counterclockwise for best focus Note The camera is...

Page 59: ...or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 59 of 84 42 Set the video properties a Place a flat white business card print side down on the calibration slide...

Page 60: ...ntific Neither this document nor the information therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4...

Page 61: ...e reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 61 of 84 e Set white balance i If the White Balance mo...

Page 62: ...e 62 of 84 i If there is a Default Registry file available click on the word Default to highlight it Check the Default load box and click Save Answer Yes to Would you like to replace the setting Then...

Page 63: ...talled OR a camera only installed and the sample aperture image was not centered on the crosshair in Microview in step 27 continue with step 44 Otherwise remove the calibration slide from the sample s...

Page 64: ...y installed adjust the z stage to focus on the calibration scale Then move the X Y stage to center the edge of the 50 m tic mark so it is positioned at the exact center of the gray crosshair in the ce...

Page 65: ...n the video calibration screen The designated center point in the video calibration screen should match what is viewed through the eyepieces Crop FOV slightly and mark edge of 50 m tic mark i If you h...

Page 66: ...ove distance marker 1 to the top of the furthest tic mark on the left side of the cropped window tic mark 30 in the illustration Move distance marker 2 to the top of the farthest tic mark on the right...

Page 67: ...y authorized by Thermo Fisher Scientific 1 4 2018 Page 67 of 84 i From the brief description drop down menu select 10X and click Set to Current Click close to exit the window j Remove the calibration...

Page 68: ...1 4 2018 Page 68 of 84 c Insert the 100 m fixed sample aperture ensuring it is properly seated and centered in either the binocular viewer OR on the red crosshair in the Microview window Refocus on t...

Page 69: ...c 1 4 2018 Page 69 of 84 f Select the Collect tab change the following parameters and click OK g From the Collect drop down menu select collect background and then click Start Collection You can choos...

Page 70: ...llect sample select the default spectrum title or enter a title click OK and leave the sample collection in preview mode l Rotate the crystal for maximum throughput see bullet points below and when co...

Page 71: ...ational alignment and save the results if applicable n Remove the sample holder with slide from the X Y stage and set aside o Remove the 100 m fixed aperture and set aside p Lower the z stage to make...

Page 72: ...act Alert plate on to the X Y stage ensuring it clicks in place Connect the contact alert cable to the side of the iN5 t Ensure that no pressure was applied to the contact alert due to the taping of t...

Page 73: ...is close to the polycarbonate sample then while watching the contact alert LED indicators continue raising the z stage until the red contact LED turns ON x Lower the z stage enough to pull the ATR ou...

Page 74: ...t steps 45u 45aa to ensure tightening did not move the crystal off center If the crystal moved loosen the plunger screws and repeat steps 45u 45aa If the crystal did not move continue with step 45dd d...

Page 75: ...whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 75 of 84 48 Configure the iS50 iN5 interface touch point if you do not have an iS50 interface continue with...

Page 76: ...stage and then focus and center the condenser as necessary 51 If not already installed insert the fixed 100 sample aperture Ensure the aperture image is centered and focused as viewed through the eye...

Page 77: ...formation therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 77 of 84 d Click Performance...

Page 78: ...distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 78 of 84 e Ensure the performance test passes If it passes continu...

Page 79: ...holder with gold or aluminum slide and focus on the slide 55 Run the Performance Test in Reflection mode a From the collect drop down menu select Experiment Setup select the Bench tab and click OPEN...

Page 80: ...distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 1 4 2018 Page 80 of 84 f Ensure the performance test passes If it passes continu...

Page 81: ...81 of 84 56 Close diagnostics window 57 Remove the sample holder and slide 58 Run ValPro If you do not have ValPro continue with step 59 a Switch the iN5 to transmission mode by moving the knob on th...

Page 82: ...under the objective Refer to the illustration in step 58j if necessary f From the OMNIC collect drop down menu select Experiment Setup select the Bench tab and click OPEN Select iN5_TransmissionMCT_De...

Page 83: ...ound and sample location when prompted k If you have an MCTA detector and Ge Tip ATR continue with step 58l otherwise continue with step 59 l Install the contact alert plate on to the X Y stage and th...

Page 84: ...er Scientific 1 4 2018 Page 84 of 84 n Remove the 32 Mil sample slide and store in the protective case 59 If a variable glass or black aperture were ordered ensure the blades move smoothly by turning...

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