3
OMNICxi Software Operation
Collect a Mosaic
Thermo Scientific
DXRxi User Guide
121
4. Open the sample compartment doors on the microscope and rotate the desired objective
to the front objective position (for easier initial focusing, start with the 10x objective).
5. Select the video calibration for this objective by setting the
Objective
option in the
OMNICxi Acquisition window.
6. Set the
to its middle position.
7. Use the microscope to locate and bring the sample into focus.
Use the joystick on the
to position the sample under the objective and the
focus control to focus the image.
You may need to adjust the brightness to bring the sample into view. If you are using
reflected light illumination and have the optional reflected light brightfield/darkfield
illuminator, the
is on the illuminator power supply. Otherwise, the
is on the microscope.
8. View the focused video image in the software.
When a sample is positioned under the objective, a live video image of the sample surface
appears in the left (video) pane in the Acquisition window. (If the image is faint or
missing, use the microscope brightness control to bring it into view.) The video image
shows approximately one field of view.
Click
Zoom To Region
to center the optical image in the right (mosaic) pane. The
optical image shows what you see when you look through the microscope eyepiece (one
field of view).
9. Change to a higher magnification objective if desired.
Rotate the objective to the front objective position on the microscope and select the video
calibration for that objective by setting the Objective option in the Acquisition window.
Note
If the objective you selected on the microscope isn’t listed under the Objective
option in the software,
for that objective.
NOTICE
Do not allow the sample to touch the objective lens. It may damage the lens
or the sample.
Tip
For quicker focusing, first watch the stage directly (not through the eyepiece)
while using the joystick to position the sample under the spot of white light. Then use
the focus control to adjust the stage height until the spot appears in focus at the
sample surface. Then look through the eyepiece while you center and focus on
features of the sample.
Tip
Make sure a distinct feature is centered under the cross hairs when viewed
through the eyepiece before you continue, especially if you plan to switch to a high
magnification (100x or higher) objective. This ensures the sample stays in the new
objective’s field of view.
Summary of Contents for DXR xi
Page 12: ...1 Introducing the DXRxi Raman Imaging System Trademarks 6 DXRxi User Guide Thermo Scientific...
Page 14: ...2 DXRxi Microscope Operation 8 DXRxi User Guide Thermo Scientific...
Page 64: ...Safety Information 58 DXRxi User Guide Thermo Scientific Microscope Safety Labels or...
Page 65: ...Safety Information Thermo Scientific DXRxi User Guide 59...
Page 66: ...Safety Information 60 DXRxi User Guide Thermo Scientific...
Page 67: ...Safety Information Thermo Scientific DXRxi User Guide 61...
Page 68: ...Safety Information 62 DXRxi User Guide Thermo Scientific...
Page 166: ...3 OMNICxi Software Operation Acquire and Analyze Data 160 DXRxi User Guide Thermo Scientific...
