CyanoFluor U
ser’s Manual
Rev. A
10
3)
Press ENTER once <ON> is selected and you’ll return to the HOME screen.
4) With the FTR Blank ON, you will be prompted to run a filtrate after every measurement
to correct for interference from DOM.
To deactivate the FTR Blank simply run through steps 1
– 3, but select <OFF> and
press ENTER. When FTR Blank is OFF, you will not be prompted to run a filtrate blank
after each measurement
and the PC and CHL Filtrate Blank will be logged as “NA”.
2.5 Sample Analysis Procedure
To make a measurement:
1) Take up a minimum of 40ccs of sample using a 60cc syringe.
2) Rinse the glass cuvette at least three times with the sample in the syringe.
3) Fill the rinsed cuvette ¾ full with sample from the syringe.
4) Use a Kim Wipe to wipe the sides of the cuvette to wipe away any moisture and/or
fingerprints.
5) Press the ON/OFF button to turn the instrument on and wait for the 5-second
countdown.
6) Press the READ button; you will be prompted to insert your sample and press read
again.
7) Making sure not to spill any sample, insert the cuvette into the sample compartment
8)
Press READ; the screen will display Measuring Please Wait…
NOTE: If FTR BLANK is ON, you will be prompted to insert a filtrate blank. See
section 2.6
“Filtering Samples for DOM Correction”, step 1.
9) Results will be displayed as:
10) Press the down arrow key to display Raw Fluorescence Values (RFU) for both PC &
CHL channels:
11) Press the down arrow key again to display FTR Blank values for both PC & CHL
channels if FTR Blank was ON and a filtrate blank was measured
PBLK FILT: 25.00
CBLK FILT: 12.00
CHL Channel Filtrate Blank RFU
PC Channel Filtrate Blank RFU
PC(RFU) 125.00
CHL(RFU) 82.18
PC Channel Raw Sample RFU
CHL Channel Raw Sample RFU
PC:CHL 1.5211
20:39 #0001
PC to Chl Ratio
Sample Number (0000
– 1000
)
Time (24 hour
clock; HH:MM)