CyanoFluor U
ser’s Manual
Rev. A
15
4.4 External Calibrations to Track Algal Abundance
Algal abundance, or other concentration estimates such as µg/L, can be calculated using
RFU values via external calibration. All that is required for an external calibration is a
standard or sample that has a known concentration and your CyanoFluor. To externally
calibrate the CyanoFluor using natural water samples:
1) Take a grab sample from your aquatic habitat
2) Measure the sample using the CyanoFluor and record the RFU value for chlorophyll
3) Use extraction methods (EPA method 445.0) to determine the actual chlorophyll µg/L
concentration of the water sample measured
4) Create a conversion factor that converts RFU to µg/L
Now all chlorophyll RFU values can be converted to µg/L concentration estimates using the
conversion factor created from the grab sample analyzed.
This kind of a correlation can also be established to convert PC RFU values to part per
billion (ppb) estimates using a PC extraction method or PC pigment which may be ordered
from
www.prozyme.com
and diluted in water to specific concentrations.
