CyanoFluor U
ser’s Manual
Rev. A
9
2.3 Calibration Check
CyanoFluor is factory calibrated. Users can check calibration stability using the Cal Check
Standard P/N 8700-951.
Cal Check Standard
The Cal Check Standard P/N 8700-951 is a sealed glass 10x10 mm cuvette containing a
specific, non-toxic fluorophore.
Do not open the Cal Check Standard
.
If the seal around
the Cal Check Standard’s cap is broken or removed, the fluorophore contained within the
cuvette may begin to degrade rendering the standard useless. Take care not to scratch the
glass cuvette as that may change the standard’s response resulting in “Failed” for the
Calibration Check. Avoid extreme temperatures as the freezing or heating of the Cal Check
Standard may cha
nge the standard’s response resulting in “Failed” for the Calibration
Check. The Cal Check Standard should be stored at room temperature (21
– 27 °C).
Procedure for Calibration Check
To check calibration stability:
NOTE: CyanoFluor should be on a flat surface when calibrating or reading standards
and samples.
1) Carefully remove the Cal Check Standard from its packaging and inspect the standard.
If the glass surfaces are not clean, use a Kim Wipe to wipe the sides of the cuvette
before inserting it into the sample compartment; wipe away any moisture and/or
fingerprints.
2)
Insert the standard into the CyanoFluor’s sample compartment and leave the
CyanoFluor lid open.
3) Press the ON/OFF button to turn the instrument on and wait for the 5-second
countdown.
4) Press the CHK STD button; results will be
displayed as “Passed” or “Failed”.
5)
If results displayed “Passed”, the instrument’s calibration has not changed, remove the
Cal Check Standard and return it to its original packaging to avoid damaging or breaking
the cuvette.
6) When finished, remove the Cal Check Standard from the sample compartment and
return it to its original packaging to avoid damaging or breaking the cuvette.
7)
If any of the results displayed “Failed”, please contact Technical Support at Turner
Designs.
2.4 Filtrate Blank
The Filtrate Blank (FTR Blank) is used to correct for interference from Dissolved Organic
Materials (DOM) in systems that have a high DOM concentration. It is recommended that
users have FTR Blank activated at all times to correct for background fluorescence, even in
low DOM environments. To activate the FTR Blank correction:
1) Press the ON/OFF button to turn the instrument ON and wait for the 5 second
countdown.
2) Press the FTR Blank button, then select <ON> using the left arrow key.
