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Setup

9

Assembly 

(cont.)

8

Gently slide the condenser up through the silver ring of the condenser 

mount  until  it  will  go  no  further  (see  figure  5).  Make  sure  that  the 

condenser is oriented as shown in figure 5.  If the condenser will not slide 

freely through the silver ring (and you’ve checked that the condenser 

set screw is backed out far enough), do not force, simply wiggle the 

condenser while lightly pushing up.
Once the condenser assembly is in place, lower the substage via the 

substage adjustment knob and tighten the condenser set screw just 

enough to prevent the condenser from spinning or sliding out.

10

All Models

Figure 5

Centering

:  The condenser must be centered in the light path to ensure proper light control.  A simple method 

for centering is as follows:  

• Rotate the nosepiece until the 10X objective is in the light path.

• Raise the substage assembly fully by turning the substage adjustment knob counter-clockwise.

• Open the aperature iris diaphragm to the largest setting by using the aperature iris diaphragm adjustment  

lever which extends from the condenser assembly.

• 

While looking into the microscope eyepieces, close the field iris diaphragm to the smallest setting by turning 

the uppermost section of the substage illuminator counter-clockwise.

• 

Closing the iris in this manner will reduce the field so that a small white hexagon is visible within a black 

field (see Figure 6A).  Focusing of the hexagon is performed by turning the coarse/fine focus controls. This 

white hexagon is the light which is passing through the field iris and should be centered in the black field.  

If not, move it to the center (see Figure 6B) by tightening and/or loosening the condenser centering knobs.

Note

:  On phase contrast models condenser centering is done with set screws (screwdriver not included) instead of  knobs.  Locations 

for two of the set screws are shown in the photo above (figure 5), and two more are on the rear of the condenser mount.  It is 

easiest to back the set screws all the way out and tighten each one slightly while checking often that the condenser is still centered.

• 

Fine tuning can be done by opening the field iris diaphragm until the white hexagon almost fills the entire 

field (see Figure 6C), and then readjusting (see Figure 6D). After centering the condenser open the field 

iris diaphragm slightly wider than the field of view.

Vertical Focusing

:  The condenser can be raised and lowered with the substage adjustment knob to focus 

the light for optimal illumination.

Aperture Adjustment

:  The light path can be adjusted with the aperature iris diaphragm adjustment lever 

located just below the condenser.  Aperture adjustments are made to induce contrast into a specimen, not 

to adjust light intensity.

1

2

3

Figure 6A

Figure 6B

Figure 6C

Figure 6D

Substage Adjustments

1

Summary of Contents for 1200CM Series

Page 1: ...Covering Models 1210CM 1211CM 1220CM 1221CM 1222CM 1223CM 1230CM 1231CM 1232CM 1233CM Operation Manual 1200CM Series...

Page 2: ...25 N A Abbe condenser Phase contrast models come with a 1 25 N A Zernike condenser with phase annulus rings for 10X 25X 40X and 100X also has a 0 setting for brightfield work All condensers are mount...

Page 3: ...0X Plan Phase Objective 1 ea 20X Plan Phase Objective 1 ea 40X Plan Phase Objective 1 ea 100X Plan Phase Objective 1 ea Dispersion Filter 1 ea Blue Filter 1 ea Green Filter 1 ea Model 1230CM Trinocula...

Page 4: ...xels for 1210 and 1220 series 1200 CDPC 3 USB DIgital Camera System 3 Megapixels for 1230 series Other Accessories Part Number Description Call for Part Numbers Eyepieces 10X 16X 20X Call for Part Num...

Page 5: ...tering Knobs Brightfield only Aperature Iris Diaphragm Adjustment Lever Fine Focus Control Substage Collector Base Stand Mechanical Stage Slide Holder Slide Holder Knobs Focusing Tension Control Knob...

Page 6: ...Fuse Holder Parts Accessories Phase Annuli Centering Knobs Phase Contrast Condenser Iris Diaphragm Adjustment Light Bulb Centering Mechanism Substage Collector Set Screw 6 Light Bulb Access Door Lock...

Page 7: ...eyetubes see figure 2 After removing the objectives from their storage containers individually install each one into the nosepiece by threading them clockwise into the threaded holes of the nosepiece...

Page 8: ...er will reduce the field so that a small white hexagon is visible within a black field see Figure 6A Focusing of the hexagon is performed by turning the coarse fine focus controls This white hexagon i...

Page 9: ...nser assembly until the two rings of light are centered upon one another see Figure 8 Once the phase rings are centered remove the phase contrast centering telescope and replace with the eyepiece The...

Page 10: ...to induce contrast into a specimen not to adjust light intensity The mechanical stage X Y controls located underneath the right hand side of the stage see figure 9 provide easy and accurate positionin...

Page 11: ...icroscope Interpupillary and Diopter Adjustment 1 2 3 Interpupillary adjustment the distance between eyepieces is made through a folding action The Seidentopf design allows for a folding adjustment wh...

Page 12: ...a adapter assembly until it is firmly secured with the camera port set screw Tighten the camera port set screw Pull the prism slider completely out to divert the image to the camera port The prism sli...

Page 13: ...Models The 100X objective which comes with this microscope must be used with immersion oil in order to maintain image quality After use the objective tip needs to be wiped clean so that no oil residu...

Page 14: ...lose the light bulb access door and screw the knob back in to lock Return the microscope to normal operating position If necessary recenter the illuminator following the instructions on page 9 1 2 7 6...

Page 15: ...r ordering information The same care instructions apply to all optical parts on this VanGuard Microscope including the substage collector and the condenser lenses All other parts can be cleaned with a...

Page 16: ...can t get enough light Step Possible Cause How to Check Result Solution For More Info 1 Variable lighting control VLC is turned down too far Visually inspect VLC turned up Go to next step VLC turned...

Page 17: ...blockage Contact dealer or VanGuard Microscopes Page 17 I can t focus on the specimen Step Possible Cause How to Check Result Solution For More Info 1 Up stop set too low Visually inspect Set too low...

Page 18: ...25 NA 8 0mm WD 1 8mm FOV Achromatic or Plan Achromatic 20X 0 40 NA 0 50mm WD 0 9mm FOV 40X 0 65 NA 0 40mm WD 0 45mm FOV 100X oil 1 25 NA 0 25mm WD 0 18mm FOV Stage Dimensions 125mm x 135mm Stage Motio...

Page 19: ...19 Notes...

Page 20: ...Printed in the USA No VGMNL070319 1200CM...

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